外源性5-氨基乙酰丙酸对NaCl胁迫下绿豆萌发及幼苗生长的影响

2020-11-23 07:38赵金玺尤颖姜凯阳张嘉祥张梅
湖北农业科学 2020年18期
关键词:丙酸乙酰绿豆

赵金玺 尤颖 姜凯阳 张嘉祥 张梅

摘要:采用单因素试验方法,以绿豆作为试材,150 mg/L浓度的NaCl模拟盐胁迫,研究不同浓度的外源5-氨基乙酰丙酸對盐胁迫下绿豆种子萌发及幼苗生长的影响。不同浓度的5-氨基乙酰丙酸处理对NaCl胁迫下绿豆的萌发及幼苗生长呈低浓度促进,高浓度抑制的作用;绿豆的发芽率和发芽势随着处理浓度的增大先升高后降低,分别在50、150 mg/L处理浓度时达到最高,在达到250 mg/L处理浓度时,发芽率和发芽势降至最低,呈显著抑制作用;幼苗期的绿豆叶面积和根长随着处理浓度增大,先升高后降低,处理浓度达到100 mg/L时二者达到最大值;随着5-氨基乙酰丙酸处理浓度的增加,绿豆幼苗叶片中丙二醛(MDA)含量呈先降低后升高的趋势,在处理浓度达到150 mg/L时最低;脯氨酸和可溶性糖含量呈先升高后降低的趋势,分别在处理浓度达到150、100 mg/L时达到最高,说明5-氨基乙酰丙酸对绿豆幼苗抗逆性具有显著影响。施用外源性5-氨基乙酰丙酸对NaCl胁迫下绿豆的萌发最佳施用浓度为100 mg/L左右,对NaCl胁迫下绿豆的幼苗生长最佳施用浓度为150 mg/L。

关键词:5-氨基乙酰丙酸;盐胁迫;绿豆;抗逆性

中图分类号:S522         文献标识码:A

文章编号:0439-8114(2020)18-0021-04

DOI:10.14088/j.cnki.issn0439-8114.2020.18.005

Effects of exogenous 5-aminolevulinic acid on the germination

and seedling growth of mung bean under NaCl stress

ZHAO Jin-xi, YOU Ying, JIANG Kai-yang, ZHANG Jia-xiang, ZHANG Mei

(Yantai Institute, China Agricultural University, Yantai  264670, Shandong, China)

Abstract: The effects of different concentrations of exogenous 5-aminolevulinic acid on seed germination and seedling growth ofmung bean under salt stress were studied by using mung bean as test material and 150 mg/L concentration NaCl as simulated salt stress. The results showed that different concentrations of 5-aminolevulinic acid promoted the germination and seedling growth of mung bean at low concentration and inhibited at high concentration under NaCl stress, the germination rate and germination potential of green beans increased first and then decreased with the increase of treatment concentration, reaching the highest concentration of 50 mg/L and 150 mg/L respectively. Exogenous 5-aminolevulinic acid played a significant inhibitory role when the concentration reached 250 mg/L. The leaf area and root length of mung bean at seedling stage increased first and then decreased with the increase of treatment concentration. When the treatment concentration reached 100 mg/L, both reached the maximum value. With the increase of 5-aminolevulinic acid concentration, malondialdehyde (MDA) decreased at first and then increased, and reached the lowest when the treatment concentration reached 150 mg/L. The content of proline and soluble sugar increased first and then decreased, and reached the highest when the treatment concentration reached 150 mg/L and 100 mg/L, respectively. The results showed that 5-aminolevulinic acid had a significant effect on the stress resistance of mung bean seedlings. The optimal concentration of exogenous 5-aminolevulinic acid on mung bean germination under NaCl stress was about 100 mg/L, and the optimal concentration for seedling growth of mung bean under NaCl stress was 150 mg/L.

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