王文婷 侯夫云 王庆美 李爱贤 郭钢 张立明
摘 要:以5个甘薯品种无菌试管苗为试材,将其在NaCl浓度为0、50、100、150 、200 mmol/L的MS 培养基上进行培养,20 d后测定各品种植株的干重、鲜重以及SOD活性。结果表明:随NaCl 浓度增大,各品种盐害程度明显加大,生长发育明显变缓;NaCl浓度为50 mmol/L时,5个品种的SOD活性都有所上升,之后随盐浓度增加分别呈现不同程度的下降;5个品种中济徐23耐盐性较强,济薯18、济黑薯1号耐盐性较差,济薯06210、徐薯18居中。
关键词:盐胁迫;甘薯;盐害;SOD活性
中图分类号:S531.038 文献标识号:A 文章编号:1001-4942(2012)11-0035-03
Initial Screening of Sweet Potato Varieties with Salt Tolerance
Wang WenTing1,2, Hou FuYun2, Wang QingMei2, Li AiXian2, Guo Gang2, Zhang LiMing2*
(1. College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China;
2. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China)
Abstract In order to screen sweet potato varieties with salt tolerance, the trial was carried out with five varieties cultured on the MS medium with 0, 50 mmol/L, 100 mmol/L, 150 mmol/L, 200 mmol/L NaCl respectively for 20 days, and then the fresh and dry weight of plants and SOD activity were investigated. The results showed that the salt injury index of different sweet potato cultivars increased significantly with the increase of NaCl concentration, and the sweet potato growth was significantly slowed. The SOD activity in 5 varieties increased under 50 mmol/L NaCl, and then reduced differently with the increase of NaCl concentration. According to these results, Jixu 23 had the highest salt tolerance; Jishu 18 and Jiheishu 1 had the worst salt tolerance; the salt tolerance of Jishu 06210 and Xushu 18 was in the middle.
Key words Salt stress; Sweet potato; Salt injury; SOD activity
甘薯是重要的粮食作物和工业原料,能够在其他粮食作物不能生长的贫瘠地块上生长,营养价值较高,在发展中国家和地区成为低投入高产出的作物。由于受环境条件与种植结构的影响,部分地区甘薯只能种植在干旱或盐渍化土壤中, 使得甘薯的产量和品质受到很大影响[1]。因此培育筛选耐盐性好的甘薯品种具有重要意义。本试验是以MS 培养基为盐害胁迫的载体,在无菌条件下对甘薯苗期的耐盐性进行研究,以期筛选出适于盐碱地区栽培的甘薯品种或品系,也可为抗盐育种提供材料。
1 材料与方法
11 试验材料
由山东省农业科学院作物研究所甘薯室提供,分别为济徐23、济薯18、济黑薯1号、济薯06210,徐薯18为对照。
12 试验方法
121 材料培养 切取大小一致茎尖顶端2~3 cm,用双蒸水冲洗后浸于70%的酒精中30 s,再用双蒸水冲洗后浸于01%升汞中10 min,最后再用双蒸水冲洗。将茎尖分别种植于MS培养基(对照)和MS+50 mmol/L NaCl(处理1)、MS+100 mmol/L NaCl(处理2)、MS+150 mmol/L NaCl(处理3)、MS+200 mmol/L NaCl(处理4)的培养基,再置于28℃、16h/8h(光照/黑暗)的培养室中培养20 d,取根、茎用于试验指标的检测,每个处理重复3次。……