SIRT1在非小细胞肺癌中的表达及相关性研究

逄旭梅△  于国华▲  张居民

[摘要] 目的 探讨沉默信息调节因子相关酶1（SIRT1）在非小细胞肺癌组织中的表达情况及其与临床病理参数之间的关系。方法 应用免疫组织化学（SABC）法检测112例经病理诊断确诊的非小细胞肺癌患者中SIRT1的表达情况，并分析其与相关临床病理参数之间的关系。 结果 SIRT1在非小细胞肺癌组织中的表达高于周围正常肺组织，差异具有统计学意义（P＜0.05），在腺癌组织中的表达明显高于在鳞癌组织中的表达（P＜0.05），并且分化程度越高，表达越好（P＜0.05），有淋巴结转移者表达明显高于无淋巴结转移者，而与年龄、性别、临床分期等无明显关系。 结论 SIRT1在非小细胞肺癌组织中的表达与病理分型、分化程度、淋巴结转移情况相关，可能成为判定非小细胞肺癌恶性程度及评估预后的生物学指标。

[关键词]非小细胞肺癌；SIRT1；免疫组织化学

[中图分类号] R734.2   [文献标识码] B   [文章编号] 2095-0616（2014）09-37-04

Expression of SIRT1 in non-small-cell lung cancer and its correlation study

PANG Xumei1  YU Guohua2  ZHANG Jumin3

1.Weifang Medical University,Weifang 261041,China;2.Department of Medical Oncology,Weifang People's Hospital,Weifang 261041,China;3. Department of Pathology, Weifang People's Hospital,Weifang 261041,China

[Abstract] Objective To investigate the expression of SIRT1 in non-small-cell lung cancer(NSCLC) tissue and its correlation with clinicopathologic features. Methods The clinicopathological data of 112 patients with non-small-cell lung cancer admitted in Weifang People's Hospital were retrospectively reviewed.Immunohistochemistry was used to examine the expression of SIRT1 in specimens of lung cancer and pericancerous tissue.The correlations of SIRT1 expression with clinicopathological features were analyzed. Results The expression of SIRT1 in cancerous tissues was higher than that in adjacent noncancerous tissues.Particularly,the expression of SIRT1 in patients with adenocarcinoma of lung was significantly higher than that in squamous cell carcinoma. Importantly，higher SIRT1 expression in cancerous tissues was correlated with higher differentiation and lymph node metastasis. However, it was not correlated with the age,gender of patients and TNM stage of the cancer. Conclusion The high expression of SIRT1 in non-small-cell lung cancer tissue is correlated with pathological type and degree of differentiation.SIRT1 may be a biological parameter to evaluate the m alignant degree of non-small-cell lung cancer and to forecast the patients prognosis．

[Key words] Non-small-cell lung cancer;SIRT1;Immunohistochemistry

原发性支气管肺癌简称肺癌，是临床最常见的恶性肿瘤之一。肺癌按组织病理学分为非小细胞肺癌和小细胞肺癌，其中最常见的类型是非小细胞肺癌（NSCLC），约占总发病人数的80%左右。沉默信息调节因子2（Sir2）是近来发现的一类具有尼克酰胺腺嘌呤二核苷酸（NAD+）依赖性的组蛋白/非组蛋白去乙酰化酶，属于第Ⅲ类组蛋白去乙酰化酶（HDAC）。SIRT1与Sir2的同源性最高，SIRT1广泛存在于生物体的胞核及胞质中，调节基因的表

达，使组蛋白、转录因子及其他蛋白修饰的赖氨酸残基去乙酰化。SIRT1通过与不同的组蛋白和非组蛋白相互作用来完成不同的功能。……