咽一方对小鼠耳肿胀及腹腔毛细血管通透性的影响

欧阳芳+薛敏菲+吴声振

【摘要】目的研究咽一方对二甲苯所致小鼠耳肿胀及醋酸所致腹腔毛细血管通透性的影响。方法采用二甲苯致小鼠耳肿胀法观察咽一方对耳廓肿胀的抑制率, 小鼠尾静脉注射伊文斯蓝, 腹腔注射醋酸造模, 测定吸光度。结果咽一方具有能显著抑制二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性的作用。结论咽一方具有较强的抗炎作用。

【关键词】咽一方；二甲苯；耳肿胀；醋酸；通透性；吸光度；小鼠

Effect of Yanyifang on ear swelling and permeability of abdomen capillary in mice model OU Yang-fang, XUE Min-fei, WU Sheng-zhen. Department of Pharmaceutics, Foshan Hospital of Traditional Chinese Medicine, Foshan 528000, China

【Abstract】Objective To investigate the effect of Yanyifang on ear swelling induced by Xylene and permeability of abdomen capillary in mice model. Methods Xylene was used to induce ear swelling, and inhibition ratio of ear swelling was observed;Yiwensilan was injected into the vessel of mice tail, then acetic acid was injected into mice abdomen to cause inflammation and determine absorbency. Results Yanyifang could significantly inhibit Xylene induced ear swelling and acetic acid induced permeability of abdomen capillary in mice model. Conclusion Yanyifang has significantly action on anti-inflammatory.

【Key words】Yanyifang; Xylene; Ear swelling; Acetic acid; Permeability; Absorbance; Mice

本院咽一方是由鱼腥草、广土牛膝、桔梗等药组成的复方, 具有清热化痰、止咳平喘、清利咽喉、养阴清火之功效, 临床证实对慢性咽炎患者的治疗具有较为独特的疗效。本文拟咽一方对二甲苯所致小鼠耳肿胀及醋酸所致腹腔毛细血管通透性的影响研究, 探讨其抗炎活性。现报告如下。

1材料与方法

1. 1动物、试剂与仪器清洁级昆明种小鼠145只, 体质量18~22 g, 均购自广州中医药大学实验动物中心。咽一方处方由鱼腥草、广土牛膝、桔梗等药材组成, 均购自广州致信中药饮片有限公司, 经本院药检室刘东文主任药师鉴定为正品。咽一方汤剂(本院煎药室制备)；吲哚美辛肠溶片(25 mg/片, 广东华南药业集团有限公司)；二甲苯(分析纯, 国药集团化学试剂公司)。紫外可见分光光度计(上海分析仪器厂), 电子天平(METTLER AE-200), 打孔器(广州市新风工业公司)。

1. 2方法

1. 2. 1咽一方对二甲苯致小鼠耳肿胀的影响实验［1, 2］取雄性小鼠75只, 体质量18~22 g, 编号、称体质量, 按体质量排序, 首先按随机数字表法随机分为五组, 即生理盐水组、吲哚美辛阳性对照组、咽一方低、中、高剂量组。……