杨春艳+刘飞+胡琼+梁云峰
摘要:用傅里叶变换红外光谱仪测定了成株期辣椒(Capsicum annuum)正常植株和根腐病植株的叶片、主根和须根3个部位的光谱。结果表明,两种植株叶片中的主要成分是蛋白质和多糖,主根和须根的主要成分是纤维素和木质素;与正常植株相比,根腐病植株叶片中蛋白质和多糖的组成发生了变化,主根中显示纤维素相对含量减少,且出现了新的蛋白质组分,须根中则显示纤维素的相对含量增加。傅里叶变换红外光谱(FTIR)可以区分根腐病对辣椒植株不同部位的影响,为辣椒根腐病的研究提供参考。
关键词:辣椒(Capsicum annuum);根腐病;傅里叶变换红外光谱
中图分类号:O657.3 文献标识码:A 文章编号:0439-8114(2014)12-2906-03
Fourier Transform Infrared Spectroscopic Study of Pepper Root Rot
YANG Chun-yan1,LIU Fei1,HU Qiong2,LIANG Yun-feng3
(1.Department of Physics, Yuxi Normal University,Yuxi 653100,Yunnan, China;
2.Department of Bioengineering, Yunnan Vocational and Technical College of Agriculture, Kunming 650212, China;
3.Agricultural Economics Management Service Centre of Yuanjiang, Yuanjiang 653300, Yunnan, China)
Abstract: Fourier transform infrared spectroscopy (FTIR) was used to obtain the spectra of leaf, taproot and fibrous roots of normal and root rot pepper plants in the adult stage. The results showed that for normal and root rot pepper plants, the main components of leaves were protein and polysaccharide, and for taproots and fibrous roots they were cellulose and lignin; for root rot plants, relative to normal plants, the structure of protein and polysaccharide in leaves has been changed, the relative content for cellulose of taproots has increased while fibrous roots decreased. The above results demonstrated that FTIR may be used to distinguish the effects of the toot rot on different parts of the pepper, which provided reference for the study of pepper root rot.
Key words: pepper(Capsicum annuum); root rot; fourier transform infrared spectroscopy
辣椒(Capsicum annuum)根腐病是由茄腐镰孢菌(Fusarium solani)引起的典型土壤传播病害。一般病田中辣椒根腐病病株率20%~40%,严重地块高达50%~80%,更有甚者造成绝产,已成为辣椒可持续生产中的一大障碍[1]。该病多发于定植后至坐果初期,发病初期植株顶部叶片稍见萎蔫,傍晚至次日早晨恢复,数日后整株枯死。病株茎部及根部皮层呈淡褐色或深褐色并腐烂,极易剥离,露出深色木质部。横切茎可见维束管变褐色,湿度大或生育后期时茎基部或根茎部腐烂[2]。对辣椒根腐病的发生、鉴定、防治及根腐菌的生物学特性已经进行过研究报道[1-4],但这些研究都没有反映病菌对染病植株化学组成的影响。
傅里叶变换红外光谱(FTIR)是一种能提供分子化学结构信息的技术,利用此技术可对样品进行定性和定量地无损分析,根据光谱的谱峰位置可以鉴定有机化合物及官能团的存在,而利用光谱的吸收强度可以定量地计算各种化学组分在样品中的相对含量[5]。……