紫山药色素醇提物的抗氧化活性研究

霍艳荣　高前欣　王振宇

摘要[目的] 采用体外试验的方法测定紫山药色素清除自由基的抗氧化性功能。[方法] 以乙醇为溶剂提取紫山药色素，AB-8大孔树脂制备纯化物，采用清除羟自由基、超氧阴离子自由基、DPPH法、测定还原力等体外试验方法研究其对自由基体系的抗氧化作用、还原力大小。[结果]试验表明，紫山药色素具有良好的抗氧化能力，纯化后抗氧化能力增强。纯化后清除羟自由基的IC50略低于VC，当纯化物浓度高于0.7 mg/ml时，其清除率高于VC；当纯化物浓度达到5.0 mg/ml时，对超氧阴离子自由基的清除率为95%，高于VC（92%）；浓度高于2.0 mg/ml时，对DPPH·的清除率与VC相当。[结论]研究可为拓展紫山药色素的应用领域提供理论依据，为天然抗氧化剂的开发人员和食品行业的技术人员提供新的参考资料。

关键词紫山药色素；醇提物；自由基；抗氧化

中图分类号S632.1文献标识码A文章编号0517-6611（2015）07-299-03

Study on Antioxidant Activity of Ethanol Extracts from Purple Yam Pigment

HUO Yan-rong1，2， GAO Qian-xin2， WANG Zhen-yu1，3*

（1. Forestry College， Northeast Forestry University， Harbin， Heilongjiang 150040； 2. Zhejiang Agricultural and Forest University， Hangzhou， Zhejiang 311300； 3.Harbin Institute of Technology， Harbin， Heilongjiang 150090）

Abstract[Objective] The function of antioxidation and scavenging free radicals of purple yam pigment was determined by using in vitro methods. [Method] Ethanol was used to extract purple yam pigment and then purified with AB-8. Hydroxyl radicals， oxygen free radicals， DPPH radicals eliminate and reducing capacity was studied by in vitro methods. [Result] The results showed that purple yam pigment has good antioxidant ability and enhanced after purification. IC50 of ·OH was slightly lower than VC and higher than it when the concentration was more than 0.7 mg/ml. The oxygen free radicals eliminate ratio was 95%， higher than VC（92%） when it was 5.0 mg/ml. The DPPH· eliminate ratio was same as VC when the concentration higher than 2.0 mg/ml. [Conclusion] The study can provide theoretical basis for expanding application field of purple yam pigment， and provide reference data for natural antioxidants developers and technical personnel of food industry.

Key wordsPurple yam pigment； Alcohol extracts； Free radicals； Antioxidant

紫山药也称“紫人参”，又名薯蓣和长芋，属薯蓣科，以其肥大的块根或圆柱状根供食用。紫山药富含薯蓣皂苷元，特别是它含有8种酚类黄酮物质的花色苷[1]。

花色苷是浆果和花朵中所看到的红色到蓝色的色素。人们对于花色苷在功能食品中的应用兴趣越来越浓，保健食品工业最近报道了花色苷的很多潜在的对健康的益处[2]，包括对冠心病，改善视觉和抗癌性，抗突变和抗炎症的效果[3-5]。

笔者采用体外试验的方法测定紫山药色素清除自由基的抗氧化性功能，为紫山药色素开辟了广阔的应用空间，为拓展紫山药色素的应用领域提供了理论依据，为天然抗氧化剂的开发人员和食品行业的技术人员提供新的参考资料。……