毕彩虹 范开业 谭建平等
摘要[目的] 为探讨不同茶树品种叶绿素组成及色泽的具体差异大小。[方法] 采用混合液法(体积比,纯丙酮∶无水乙醇∶蒸馏水=4.5∶4.5∶1.0)及色差计法,对梅占、福选9号2个品种的叶绿素组成及干茶色泽、汤色、叶底色泽进行测定。[结果] 梅占的叶绿素a、叶绿素b、叶绿素总量均高于福选9号,且差异显著;干茶、茶汤、叶底色差测定表现为梅占品种的亮度不如福选9号,但梅占相对绿度重,福选9号相对黄度重。[结论]不同茶树品种间叶绿素含量及组成存在差异,且差异的大小直接影响到绿茶的成茶色泽,梅占的成茶色泽要优于福选9号。
关键词梅占;福选9号;叶绿素;色泽
中图分类号S571.1文献标识码
A文章编号0517-6611(2015)31-022-01
Chlorophyll and Color Comparison of Two Tea Varieties of Meizhan and Fuxuan 9
BI Caihong1,FAN Kaiye1,TAN Jianping2 et al
(1.Linyi Fruit and Tea Technology Extension and Service Center,Linyi, Shandong 276000; 2.Linyi Agricultural Comprehensive Law Enforcement Detachment,Linyi, Shandong 276000)
Abstract [Objective] The research aimed to study chlorophyll composition and color specific differences about different teatree varieties. [Method] Chlorophyllcomposition and dryteacolor,liquor color,infusion color of Meizhan and Fuxuan 9 were tested and analyzed by using the method of mixture (volume ratio, acetone∶ anhydrous ethanol∶distilled water=4.5∶4.5∶1.0) and colorimeter. [Result] Chlorophyll a, chlorophyll b and total chlorophyll of Meizhan were higher than those of Fuxuan 9,and the brightness and the yellow ratio were better than those of Meizhan, but the green ratio of Meizhan was more.
[Conclusion]Chlorophyll content and the composition have differences among different tea varieties. The differences directly affect the tea color. Tea color of Meizhan is better than Fuxuan 9.
Key words Meizhan(Camellia sinensis);Fuxuan 9(Camellia sinensis);Chlorophyll;Color
色澤是辨别茶叶品质的主要内容之一。绿茶的色泽要求“三绿”,即干茶、汤色和叶底三绿[1]。色泽又是茶叶相关化学成分的综合反映,而叶绿素是绿茶中的主要色素,主要由蓝绿色的叶绿素a和黄绿色的叶绿素b所组成[2]。笔者对梅占、福选9号2个品种的叶绿素组成和色泽进行比较,以期找出其中的具体差异。
1材料与方法
1.1试验材料
选用重庆巴南二圣茶园同等管理水平的梅占、福选9号两个品种的一芽二叶为原料。2015年5月27日严格按标准采摘,制绿茶样。
1.2试验方法
1.2.1叶绿素组成的测定。将茶样磨成细末,精确称取茶样0.3 g移入25 ml棕色容量瓶中,用混合液(体积比,纯丙酮∶无水乙醇∶蒸馏水=4.5∶4.5∶1.0)定容,在常温暗处避光条件下萃取24 h,2 h振荡1次,取上清液,以混合液为空白对照,用UV2102 PC型紫外可见分光光度计测定光密度,计算叶绿素a(chla)、叶绿素b(chlb)及叶绿素总量(chla+chlb)的含量[3-4]。……