异丙肾上腺素对婴儿血管瘤内皮细胞影响的体外研究

异丙肾上腺素对婴儿血管瘤内皮细胞影响的体外研究

聂晶1， 帕提古丽·买买托合提2， 朱雅琳1

(1新疆医科大学第一附属医院皮肤科， 乌鲁木齐830054；2新疆和田地区墨玉县人民医院皮肤科， 新疆和田848000)

摘要：目的探讨不同浓度异丙肾上腺素在体外对婴儿血管瘤内皮细胞生长的影响及β-肾上腺素受体在婴儿血管瘤发病机制中的作用。方法将增殖期婴儿血管瘤内皮细胞分为空白组(不含药物培养基)及异丙肾上腺素低浓度组(5、10、20 mg/L)与高浓度组(50、100、200 mg/L)。药物作用1~10 d采用MTT法分别测定各组细胞的生长曲线。结果低浓度组24~96 h对细胞生长呈现刺激作用；96 h时,低浓度组与空白对照组的吸光度值差异有统计学意义(P<0.05)。而高浓度组在24~96 h对细胞生长呈现抑制作用，与空白对照相比，高浓度组细胞的吸光度值差异有统计学意义(P<0.05)，并且24~96 h浓度越高，作用越强。结论β-肾上腺素受体在婴儿血管瘤发病中有重要作用;异丙肾上腺素对于体外培养的增殖期血管瘤内皮细胞的刺激作用与浓度相关。

关键词:婴儿血管瘤; 血管内皮细胞; 异丙肾上腺素; β-肾上腺素受体

中图分类号：R969.4文献标识码：A

doi:10.3969/j.issn.1009-5551.2015.07.010

[收稿日期：2015-02-07]

基金项目：新疆地方病分子生物学实验室开放课题基金(XJDX0208-2011-06)

作者简介：祖力卡尔江·阿合买提，男(维吾尔族)，硕士，研究方向：龋病学的防治研究。

The effect of isoproterenol on hemangioma endothelial cells in vitro

NIE Jing1, Patiguli Maimaituoheti2，ZHU Yalin1

(1DepartmentofDermatology,TheFirstAffiliatedHospitalofXinjiangMedicalUniversity，

Urumqi830054,China;2DepartmentofDermatology,XinjiangHetian,People′sHospital,

Hetian848000,China)

Abstract:ObjectiveTo investigate the different concentrations of isoproterenol in vitro effects on infant hemangioma endothelial cell growth and to study the function of beta adrenergic receptors in the pathogenesis of infantile hemangioma. MethodsInfantile hemangioma endothelial cells in proliferating phase were divided into three groups, the blank group (without drug culture medium), low concentration group(5, 10, 20 mg/L) of isoproterenol and high concentration group (50, 100, 200 mg/L). The growth curve of cells in each group were measured after a certain time by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method. ResultsLow concentration group showed stimulation affection on cell growth within 24-96 hours; and in the high concentration group, cell growth were inhibited within 24-96 hours, and the hiher concentration, the stronger the effect within 24-96 hours. ConclusionBeta adrenergic receptors play an important role in the pathogenesis of infantile hemangioma;stimulation effection of isoproterenol is related with concentration of drug for in vitro culture of hemangioma endothelial cells.

Key words： infant hemangioma; endothelial; isoproterenol; beta adrenergic

血管瘤是由胚胎期血管网增生所形成。在胚胎早期，原始血管是一种单纯由内皮细胞组成的管状物，它们在间质中形成一个致密的网。随着各器官和组织的发育，原始血管网逐渐分化成为与它们之间联系的许多血管丛。根据原始血管内的流量和压力，形成供应血管(动脉)或引流血管(静脉)。体表和皮下组织的血管网易发生不规则的局部增生，日后即成为血管瘤。血管瘤是一种先天性发育异常，属于错构瘤性质，而非真性肿瘤，具有畸形和肿瘤的双重特性。……