双氢青蒿素对小鼠肝纤维化形成的影响 ※

双氢青蒿素对小鼠肝纤维化形成的影响※

李校天郭永泽△

(河北工程大学附属医院消化内科，河北邯郸056002)

【摘要】目的探讨双氢青蒿素(dihydroartemisinin，DHA)对小鼠肝纤维化形成的影响。方法将24只雄性C57BL/6野生型小鼠随机分为3组，即对照组、模型组及治疗组，每组各8只。模型组及治疗组小鼠予2 μL/g体质量四氧化碳(CCl4)橄榄油溶液腹腔注射，每3 d 1次，共注射12次，制备肝纤维化模型。对照组小鼠予等量、等次橄榄油溶剂腹腔注射。治疗组于腹腔注射CCl4橄榄油溶液8次后，予20 μg/g体质量DHA原药灌胃给药，同时对照组及模型组小鼠予等容积含二甲基亚砜(DMSO)0.9%氯化钠注射液灌胃。均每日1次，直至处死前 1 d。最后一次注射后48 h，所有小鼠处死取肝组织，肉眼观察，并进行苏木精—伊红(HE)染色和天狼星红染色，然后光学显微镜观察。结果模型组及治疗组肝组织病理证实肝纤维化模型建立成功，治疗组小鼠肝小叶破坏、肝细胞变性及纤维组织增生程度均低于模型组，天狼星红染色面积较模型组明显下降，差异有统计学意义(P<0.05)。 结论DHA能够改善CCl4诱导的小鼠肝纤维化程度。

【关键词】青蒿素；肝硬化；四氯化碳；疾病模型，动物；小鼠；实验

doi：10.3969/j.issn.1002-2619.2015.00.000

【中图分类号】R285.5；R284.2；R657.31；R285.5

【文献标识码】A

【文章编号】1002-2619(2015)03-0400-03

Abstract【】ObjectiveTo investigate the effect of dihydroartemisinin (DHA) on hepatic fibrosis mice.Methods24 male C57BL/6 wile-type mice were randomly divided into control group, model group and treatment group, 8 mice in each group. Liver Fibrosis model in model group and treatment group were induced by intraperitoneal injection of carbon tetrachloride (CCl4) diluted with olive oil once every third day for 12 times. Mice in control group received intraperitoneal injection of olive oil at a same dose and same times as other groups. After 8 times of injection, mice in treatment group received 20 μg/g of DHA via gastrogavage, mice in other two groups received isasteric dimethyl sulfoxide diluted with sodium chloride injection by gastrogavage. Mice in all groups were sacrificed 48 hours after the final injection of CCl4. The changes of liver tissue were observed by hematoxylin, eosin and sirius red staining. ResultsModel foundation of liver fibrosis in model group and treatment group were confirmed by histological analysis. The degree of liver lobular damage, liver cell degeneration and fibrous tissue hyperplasia in treatment group were lower than those in model group. The percentage of sirius red staining area in treatment group was less than that in model group(P<0.05). ConclusionDHA can improve degree of carbon tetrachloride-induced liver fibrosis in mice.

通讯作者：△河北工程大学附属医院消化内科，河北 邯郸 056002

作者简介：李校天(1964—)，男，教授，硕士。研究方向：慢性肝病、肝纤维化的诊疗。

收稿日期：(2014-12-12)

Effects of dihydroartemisinin on hepatic fibrosis miceLIXiaotian,GUOYongze.DepartmentofGastroenterology,TheHospitalAffiliatedtoHeibeiEngineeringUniversity,Hebei，Handan056002

【Key words】Dihydroartemisinin; Liver fibrosis; Carbon tetrachloride; Mice; Disease model; Animal; Experiment

※ 项目来源：2013年邯郸市科学技术研究与发展计划(第二批)(编号：1323108088-5)

肝纤维化是各种慢性肝病进展为肝硬化的必经阶段。因此，逆转或阻止肝纤维化是目前研究的热点。但是至今尚未找到理想的抗肝纤维化药物。……