川芎嗪对PGcABCG-2蛋白影响的研究

川芎嗪对PGcABCG-2蛋白影响的研究

杨栋1，张培彤1*，王耀焓1，郭秀伟1，马雪曼2

(1.中国中医科学院广安门医院肿瘤科,北京 100053；2.北京中医药大学，北京 100029)

摘要：目的研究川芎嗪对肺癌PG-BE1干细胞样细胞ABCG-2蛋白表达的影响。方法采用无血清成球培养法分选PG-BE1中的干细胞样细胞亚群，并检验其干性,采用MTT法检测PG-BE1干细胞样细胞与原代细胞对常用化疗药的耐药性。采用Western blot法检测PG-BE1干细胞样细胞与原代细胞ABCG-2蛋白表达及不同浓度川芎嗪对ABCG-2蛋白表达的影响。结果PG-B1干细胞样细胞的干细胞相关蛋白表达及克隆灶形成数目与原代细胞差异有统计学意义；PG-BE1干细胞样细胞化疗药耐药性强于原代细胞；PG-BE1干细胞样细胞ABCG蛋白表达高于原代细胞；川芎嗪对ABCG蛋白的表达有抑制作用。结论无血清成球培养法可有效富集PG-BE1干细胞样细胞，PG-BE1干细胞较其原代细胞有更强的耐药性，这可能与其高表达ABCG-2蛋白有关,川芎嗪对PG-BE1干细胞样细胞ABCG-2蛋白有抑制作用。

关键词：细胞耐药;肺癌干细胞;ABCG-2;川芎嗪

DOI:10.13463/j.cnki.jlzyy.2015.08.024

中图分类号：R273文献标志码： A

文章编号：1003-5699(2015)08-0833-04

基金项目:国家自然科学基金面上项目(81173450)；国家自然科学基金青年项目(81302963)。

作者简介:杨栋(1985-)，男，博士研究生，医师，主要从事肺癌血瘀证研究。

收稿日期:(责任编辑：赵玉芝2014-03-06)

*通信作者:张培彤，电话-13910678101，电子信箱-zhangpeitong@sohu.com

Study on the effect of TMP on the expression of PGcABCG-2 protein

YANG Dong1,ZHANG Peitong1*,WANG Yaohan1,GUO Xiuwei1,MA Xueman2

(1.Department of Oncology,Guang’anmen Hospital,China Academy of Chinese Medical Science,Beijing 100053,China;

2.Beijing University of Chinese Medicine,Beijing 100029,China)

Abstract:ObjectiveTo study on the effect of TMP,which is the effective composition of Chuanxiong,on the expression of ABCG-2 protein of high metastatic human lung cancer(PG-BE1) cells.MethodsFirst of all,we sort the cancer stem cell(CSC) subgroup from the high metastatic human lung cancer cells(PG-BE1) by serum free sphere culture method and examine the properties of stem cells.MTT is used to exam the resistance to common chemotherapy medicine of the PG-BE1 CSC like cells and parental cells. Then we use the western blot experiment to verify the expression of ABCG-2 protein in the PG-BE1 CSC like cells and parental cells and the influence of different concentration of TMP on the expression of ABCG-2 protein.ResultsThe number of clone formation and the expression of CSC related protein show significant differences.PG-BE1 CSC cells have stronger chemotherapy resistance than parental cells.The expression of ABCG-2 protein is higher than parental cells.TMP can inhibit the expression of ABCG-2 protein.ConclusionThe serum free sphere culture method can effective enrich the CSC like cells and the PG-BE1 CSC like cells show stronger chemothrepy resistance than their parental cells.High expression of ABCG-2 protein may contribute to the themotherapy resistance.TMP can inhibit the expression of the ABCG-2 protein of PG-BE1 CSC like cells.

Keywords:chemotherapy resistance;lung cancer stem cells;ABCG-2;Ligustrazine

肿瘤多药耐药(multidrug resistance,MRD)是肿瘤化疗失败和疗后复发的关键因素，逆转MRD是增强化疗疗效和减少肿瘤复发的关键。关于MRD的产生，学者多从细胞药物外排能力增强、细胞靶标变异、DNA损伤修复能力增强及凋亡逃避等角度解读。……