干旱对冬小麦叶片气孔、活性氧和光合作用的影响

干旱对冬小麦叶片气孔、活性氧和光合作用的影响

李思，张莉，姚雅琴

(西北农林科技大学 生命科学学院，陕西 杨凌712100)

摘要：通过盆栽控水方式，以冬小麦小偃22叶片为实验对象，利用生理指标测定、 光合测定和H2DCFDA荧光标记等相结合的方法，研究了4个水分处理下(正常供水CK；轻度亏水LS；中度亏水MS；重度亏水SS)，冬小麦叶片中活性氧含量、丙二醛(MDA)含量、活性氧清除系统的相关保护酶活性及水分胁迫情况下过氧化氢(H2O2)在小麦叶片内的分布情况，同时，还探讨了不同水分胁迫处理对小麦气孔的密度及光合性能指标的影响.结果表明，随着水分胁迫程度的加剧，小麦叶片内H2O2和MDA含量逐渐增加；叶片的气孔密度增加,其中重度胁迫较正常灌溉情况气孔密度增加了44.12%；而光合性能指标(蒸腾速率、光合速率、气孔导度和叶肉细胞间CO2浓度)随水分胁迫的加剧呈下降的趋势. H2O2在叶表皮中的含量随水分胁迫加剧呈增加趋势,且在重度胁迫情况下分布较其他情况更广.本实验为小麦抗旱机理的研究提供了实验依据，为解决旱区农业作物种植问题提供了一定的理论指导.

关键词：小麦；水分胁迫；活性氧；气孔；光合特性

DOI：10.3969/j.issn.1000-1565.2015.05.008

中图分类号：Q945.78；S512.1文献标志码：A

收稿日期:2015-04-17

基金项目：国家自然科学基金资助项目(509073)

Effects of different water stress on active oxygen，stoma and

photosynthesis characteristics of wheat

LI Si，ZHANG Li，YAO Yaqin

(College of Life Science, Northwest A & F University, Yangling 712100, China)

Abstract：By means of water control in pot culture, winter wheat (Triticum aestivum L. ) Xiaoyan 22 leaves are used as the experimental material to be tested by methods of physiological indicator detection， photosynthesis measurement and H2DCFDA flourescent dye to figure out the active oxygen amount, malonaldehyde content and distribution of hydrogen peroxide in winter wheat leaves under four water stress conditoins(Control as CK；Light drought stess as LS；Mediate drought stess as MS；Severe drought stess as SS). Meanwhile, the density of winter wheat stoma as well as hydrogen peroxide indexes under water stress has also been explored in this experiment. The results are as follows: with the water stress becoming severer, hydrogen peroxide and malonaldehyde content as well as the density of winter wheat stoma gradually increase, the density of stoma under severe drought conditions shows a rise of 44.12% than that

第一作者：李思(1989-)，女，河北正定人，西北农林科技大学在读硕士研究生，主要从事植物抗旱生理研究.

E-mail：445613242@qq.com

通信作者：姚雅琴(1957-)，女，陕西澄城人，西北农林科技大学教授，主要从事植物逆境……