子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

刘洪正，　王玉亮

子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

刘洪正1，王玉亮2

(1. 天津市滨海新区汉沽中医医院检验科，天津 300480；

2. 天津市第一中心医院检验科卫生部危重病急救医学重点实验室，天津 300192)

摘要：目的探讨子宫内膜异位症与Fas介导的腹腔液单个核细胞凋亡的关系。方法选取手术后病理证实为子宫内膜异位症的患者55例作为子宫内膜异位症组，以非子宫内膜异位症患者52例作为对照组，分离腹腔液单个核细胞，应用实时逆转录聚合酶链反应(RT-PCR)测定Fas mRNA的表达水平，应用流式细胞术定量检测Fas表达细胞百分率。结果子宫内膜异位症组腹腔液单个核细胞Fas mRNA及Fas表达细胞百分率明显高于对照组(P<0.05)，Fas mRNA及Fas表达细胞百分率在子宫内膜异位症组Ⅰ～Ⅱ期与Ⅲ～Ⅳ期中表达差异无统计学意义(P>0.05)。结论子宫内膜异位症患者腹腔液单个核细胞Fas表达异常，对子宫内膜异位症具有一定的作用。

关键词：Fas；单个核细胞；腹腔液；子宫内膜异位症

Expression of Fas in peritoneal fluid mononuclear cells among endometriosis patients and its significanceLIUHongzheng1，WANGYuliang2

.(1.DepartmentofClinicalLaboratory,HanguChineseMedicineHospitalofTianjinBinhaiNewArea,Tianjin300480,China; 2.DepartmentofClinicalLaboratory,TianjinFirstCentralHospital,KeyLaboratoryforCriticalCareMedicineoftheMinistryofHealth,Tianjin300192,China)

Abstract:Objective To investigate the relationship of Fas expression in peritoneal fluid mononuclear cells and endometriosis. MethodsA total of 55 patients with histopathologically diagnosed endometriosis after operation were enrolled as endometriosis group, and 52 patients without endometriosis were enrolled as control group. The peritoneal fluid mononuclear cells were isolated, the expression levels of Fas mRNA were determined by reverse transcriptase-polymerase chain reaction(RT-PCR), and the percentage of Fas expression cells were quantitatively detected by flow cytometry. ResultsFas mRNA and the percentage of Fas expression cells were significantly higher in endometriosis group than those in control group(P<0.05). There was no statistical significance between stage Ⅰ-Ⅱand stage Ⅲ-Ⅳ(P>0.05). ConclusionsThe abnormal Fas expression in peritoneal fluid mononuclear cells may play an important role in endometriosis.

Key words:Fas; Mononuclear cell; Peritoneal fluid; Endometriosis

细胞凋亡是机体清除感染、变异、衰老的细胞，维持自身正常生理平衡的一种调节方式。Fas/FasL是引起细胞凋亡的重要通路之一，也是杀伤性T淋巴细胞杀伤靶细胞的三大途径之一[1]。Fas/FasL系统对于正常人体的发育、器官分化、免疫调节等一系列重要生理功能都是必不可少的，其异常与肿瘤、自身免疫疾病、移植排斥等发生、发展有密切关系[2-4]。子宫内膜异位症多发生于育龄期妇女，近年来发病有明显上升趋势[5]，其发病机制尚不明确。近年研究发现，子宫内膜异位症患者与正常人内膜细胞的凋亡率存在显著差异，异位的子宫内膜细胞表现过度增殖及凋亡异常可能是子宫内膜异位症发生、发展的重要原因之一[6]。……