雪莲通脉丸对冠心病气虚血瘀证小鼠血管内皮细胞功能相关指标ET-1、NO、ACE的影响

秦　汉，胡志希*，李　琳，吕　佳，杨　杨，张　易（湖南中医药大学，湖南长沙410208）



雪莲通脉丸对冠心病气虚血瘀证小鼠血管内皮细胞功能相关指标ET-1、NO、ACE的影响

秦汉，胡志希*，李琳，吕佳，杨杨，张易
（湖南中医药大学，湖南长沙410208）

〔摘要〕目的观察雪莲通脉丸对冠心病气虚血瘀证小鼠内皮素-1（ET-1）、一氧化氮（NO）和血管紧张素转换酶（ACE）的调节作用。方法建立冠心病气虚血瘀证小鼠模型，随机分为空白对照组、模型对照组、地奥心血康组和雪莲通脉丸组。给药4周后取小鼠血清，ELISA试剂盒测定ET-1、NO和ACE浓度。结果与模型组比较，雪莲通脉丸组NO浓度显著升高，差异具有统计学意义（P＜0.01）；ET-1浓度降低，差异具有统计学意义（P＜0.05）；ACE浓度显著降低，差异具有统计学意义（P＜0.01）。与地奥心血康组比较，雪莲通脉丸组NO浓度显著升高，ACE浓度显著降低，差异具有统计学意义（P＜0.01）。结论冠心病气虚血瘀证的发生可能与血管内皮细胞的功能指标具有相关性。其中，冠心病气虚血瘀证可能与血清ET-1、ACE浓度呈正相关、与血清NO浓度呈负相关。雪莲通脉丸对冠心病气虚血瘀证小鼠血管内皮细胞的功能指标具有调节作用，其机制可能与升高血清NO浓度、降低ET-1、ACE浓度有关，且与地奥心血康组比较在改善血清NO、ACE方面具有一定优势。

〔关键词〕雪莲通脉丸；气虚血瘀证；冠心病；内皮素-1；一氧化氮；血管紧张素转换酶；天山雪莲；西洋参；肉苁蓉

Effects of Xuelian Tongmai Pill on Vascular Endothelial Cell Function-Related Marker ET-1, NO and ACE in Coronary Heart Disease Mice Models with Qi Deficiency and Blood Stasis Syndrome

QIN Han, HU Zhixi*, LI Lin, LV Jia, YANG Yang, ZHANG Yi
（Hunan University of Chinese Medicine, Changsha, Hunan 410208, China）

〔Abstract〕Objective To observe the regulatory effects of Xuelian Tongmai Pill（XTP）on endothelin-1（ET-1）, nitric oxide（NO）and angiotensin converting enzyme（ACE）in coronary heart disease mice models with Qi deficiency and blood stasis syndrome. Methods Coronary heart disease mice models with Qi deficiency and blood stasis syndrome were established and then distributed to blank control group, model control group, Di'ao Xinxuekang group and XTP group. Serum of each group were picked after four weeks. The concentration of ET-1, NO and ACE were measured by ELISA. Results Compared with model group, concentration of NO was highly elevated with statistical significance（P＜0.01）; ET -1 was decreased with statistical significance（P＜0.05）; ACE was prominently decreased with statistical significance（P＜0.01）. Compared with Di'ao Xinxuekang group, the concentration of NO was highly increased, while ACE was significantly decreased, the diffrences have statistical significance（P＜0.01）. Conclusion Morbidity of coronary disease with Qi deficiency and blood stasis syndrome may be related to the indicators of vascular endothelial cell function. Qi deficiency and blood stasis syndrome Serum concentration of showed positive relations with ET-1 and ACE, while negative relation with NO concentration. XTP showed regulatory function on the indicator of vascular endothelial cell function. The mechanism may be related to the increasing of serum concentration of NO and decreasing of ET-1 and ACE. In addition, XTP showed more advantages inimproving serum NO and ACE concentration.

〔Keywords〕Xuelian Tongmai Pill; Qi deficiency and blood stasis syndrome; coronary heart disease; endothelin-1; nitric oxide; angiotensin converting enzyme；Saussurea involucrate; American ginseng; Cistanche salsa

冠心病是严重危害人类健康的常见病，其发病率、病死率、住院率呈迅速上升趋势[1]。冠心病始于冠状动脉粥样硬化，继而产生管腔狭窄或闭塞，导致心肌缺血缺氧。……