银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究

彭　志，卢芳国，屈金艳，李　玲，范伏元*，那婧婧，李湘兰（.湖南中医药大学，湖南长沙4008；.湖南安邦制药有限公司，湖南长沙40008；.湖南中医药大学第一附属医院，湖南长沙40007）



银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究

彭志1，卢芳国2，屈金艳3，李玲2，范伏元3*，那婧婧1，李湘兰1
（1.湖南中医药大学，湖南长沙410208；2.湖南安邦制药有限公司，湖南长沙410008；3.湖南中医药大学第一附属医院，湖南长沙410007）

〔摘要〕目的研究银黄清肺胶囊含药血清体外抑制呼吸道合胞病毒（RSV）的作用。方法采用观察CPE及MTT法，观察在银黄清肺胶囊含药血清干预下，感染RSV Hep-2细胞的CPE及光密度值，从而比较不同给药方式（预防给药、治疗给药、直接杀灭）及不同药物剂量下银黄清肺胶囊对RSV的抑制作用。结果在三种给药方式中，与相应浓度空白血清比较，各浓度银黄清肺胶囊与利巴韦林片含药血清OD值均明显增高（P＜0.05）；其中稀释度为3.13%的利巴韦林片含药血清和稀释度为6.25%的银黄清肺胶囊含药血清相比本组其他浓度含药血清OD值均明显增高（P＜0.05）。结论银黄清肺胶囊含药血清对RSV有直接灭活作用，对RSV侵入Hep-2细胞有阻断作用，对RSV在Hep-2细胞内增殖有抑制作用。其中稀释度为3.13%的利巴韦林含药血清和稀释度为6.25%的银黄清肺胶囊含药血清相比本组其他浓度含药血清体外抗RSV活性强。

〔关键词〕呼吸道合胞病毒；银黄清肺胶囊；含药血清；抗病毒；葶苈子；炙麻黄；苦杏仁

Experimental Research of Yinhuang Qingfei Capsules Containing Serum on the Respiratory Syncytial Virus in Vitro

PENG Zhi1，LU Fangguo2，QU Jinyan3，LI Ling2，FAN Fuyuan3*，NA Jingjing1，LI Xianglan1
（1. Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; 2. Hunan Anbang Pharmaceutical Co. Ltd., Changsha, Hunan 410208, China; 3. The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China）

〔Abstract〕Objective To study Yinhuang Qingfei capsules containing serum on the respiratory syncytial virus in vitro. Methods The cytopathic effect（CPE）and optical density（OD）of respiratory syncytial virus（RSV）infection Hep-2 cells, which were intervened by Yinhuang Qingfei capsules, were by CPE and MTT methods. The inhibition effects of Yinhuang Qingfei capsules on RSV with different doses and different administration methods（drug prevention, treatment, directly kill）were observed. Results Compared with the corresponding concentration of blank serum, the serum OD values in Yinhuang Qingfei capsule and ribavirin tablets containing serum groups were significantly increased（P＜0.05）; Compared with other oncertrations of the same groups, the OD valiues of ribavirin with 3.125%dilution and Yinhuang Qingfei with 6.125%dilution were increased obviuosly（P＜0.05）. Conclusion Yinhuang Qingfei capsule showed directly inactivation effect on RSV, blocking effect on RSV invade Hep-2 cells, proliferation inhibition on RSV in Hep-2 cell, and the ribavirin with 3.125%dilution and Yinhuang Qingfei with 6. 25%dilution showed strongest anti-RSV effect in vitro.

〔Keywords〕respiratory syncytial virus, Yinhuang Qingfei capsule; drug serum; antiviral; pepperweed seed; prepared ephedra; bitter almond kernel

呼吸道合胞病毒（respiratory syncytial virus, RSV）主要引起婴幼儿下呼吸道感染，即喘息性毛细支气管肺炎，较大儿童和成人主要引起上呼吸道感染。……