血清铁生物剂量计的可行性研究

谢丽华 张晓红 胡晓丹 陈 峰 张泽群 李 群 张海黔（南京航空航天大学 南京 210016）



血清铁生物剂量计的可行性研究

谢丽华 张晓红 胡晓丹 陈 峰 张泽群 李 群 张海黔
（南京航空航天大学 南京 210016）

摘要研究人体外周血血清经急性γ射线照射后血清铁的浓度变化，探讨其作为生物剂量计的可行性。人体血清经不同剂量γ射线辐照，采用酶联免疫吸附试验 (Enzymes linked immunosorbent assay, ELISA) 法定量检测血清铁的浓度，建立血清铁浓度与吸收剂量之间的剂量−效应关系。结果显示，血清铁浓度随吸收剂量升高，血清铁浓度与吸收剂量成线性平方关系：男性[y=30 676.5+2 383.5x–202.7x2(r=0.99)]；女性 [y=26 974.4+ 1 611.8x–141.3x2(r=0.98)]。4 Gy剂量时间−效应关系研究发现离体血清铁在2 d内维持稳定。利用建立的线性平方剂量−效应曲线估算的吸收剂量与双盲剂量(1、2、4 Gy)接近，估算值的95％置信区间较窄。研究结果提示血清铁具有重建人体吸收剂量的潜能。

关键词生物剂量计，血清铁，γ射线

(BK20131355)和江苏高校优势学科建设工程项目资助

第一作者：谢丽华，女，1989年2月出生，2013年毕业于南华大学，现为南京航空航天大学在读硕士研究生，从事生物剂量计研究

Supported by National Defense Basic Scientific Research Project (B252013307), National Nature Science Foundation of China (11575086, 31400721), Nature Science Foundation of Jiangsu Province (BK20131355) and Priority Academic Program Development of Jiangsu Higher Education Institutions

First author: XIE Lihua, female, was born in February 1989 and graduated from University of South China in 2013. Now she is a master candidate of Nanjing University of Aeronautics and Astronautics, majoring in biological dosimeter

Received 27 September 2015, accepted 10 November 2015

Development of a new biodosimetry of serum iron

XIE Lihua ZHANG Xiaohong HU Xiaodan CHEN Feng ZHANG Zequn LI Qun ZHANG Haiqian
(Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China)

ABSTRACT The aim is to study the serum iron changes in vitro serum of human peripheral blood irradiated by Co-60 γ-rays and explore its feasibility as a biodosimetry. To establish the dose-response relationship, the concentration of serum iron in γ-rays irradiated serum was detected using ELISA (Enzymes linked immunosorbent assay). The results demonstrated that serum iron level increased with absorbed dose, and the dose-response curve of serum iron concentration followed the linear-quadratic model: male [y=30 676.5+2 383.5x–202.7x2(r=0.99)], female [y=26 974.4+ 1 611.8x–141.3x2(r=0.98)]. Time-response relationship of 4 Gy demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve, the absorbed dose was close to the double-blind dose (1, 2 and 4 Gy), and the 95％ confidence interval of the estimated value was narrow. Therefore, the serum iron has the potential to be a new biodosimetry to reconstruct human exposure dose.

KEYWORDS Biological dosimeter, Serum iron, γ-rays

CLC Q691, TL72

生物剂量计是通过分析和检测人体生物材料（如组织、细胞、DNA、蛋白质等）在电离辐射后发生的某个方面的改变，利用这种改变建立剂量−效应关系，并通过这种量效关系来刻度、分析和评估生物吸收剂量的一类生物标志物或相关分析方法[1]。由于其忠实性和可重复性，生物剂量计在突发性核事故和放射性事故伤员的分类和救治过程中的作用非常重要[2]。……