高效液相色谱法同时检测叶面肥料中4种植物生长调节剂的研究

杨　一1，张　娟2，朱海荣2

(1.上海化工研究院　上海　200062； 2.山东省产品质量检验研究院　山东济南　250100)



高效液相色谱法同时检测叶面肥料中4种植物生长调节剂的研究

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杨一1，张娟2，朱海荣2

(1.上海化工研究院上海200062； 2.山东省产品质量检验研究院山东济南250100)

摘要通过试验，建立了同时测定叶面肥料中氯吡脲、烯效唑、萘乙酸和脱落酸4种植物生长调节剂的高效液相色谱分析方法。试验结果表明：在样品采用甲醇提取、以甲醇-0.1%甲酸水溶液作为流动相、采用梯度洗脱、进样量10 μL、柱温35 ℃的条件下，测试样品的测定结果在1.0～100.0 mg/L范围内线性关系良好，检出限为0.1 mg/L；在0.05%，0.50%和5.00%添加水平上，方法的平均回收率在90.0%～105.0%之间，相对标准偏差(RSD)<5%(n=6)；该方法通用性强、选择性好、灵敏度高，可满足叶面肥料中氯吡脲、烯效唑、萘乙酸和脱落酸4种植物生长调节剂同时检测的要求。

关键词高效液相色谱法叶面肥料氯吡脲烯效唑萘乙酸脱落酸

Study of Determination of Four Plant Growth Regulators in Foliar Fertilizers Simultaneously with High Performance Liquid Chromatography

YangYi1, Zhang Juan2, Zhu Hairong2

(1.Shanghai Research Institute of Chemical IndustryShanghai200062；2.Shandong Institute for Product Quality InspectionShandong Jinan 250100)

AbstractThrough experiment, an analysis method for determination of forchlorfenuron, uniconazole, naphthylacetic acid and abscisic acid in foliar fertilizers at the same time with high performance liquid chromatography is established. Experimental results show that under conditions that samples are extracted with methanol, methanol and 0.1% formic acid aqueous solution are used as mobile phase, gradient elution is used, the injection volume of the sample is 10 μL and column temperature is 35 ℃, in the range of 1.0～100.0 mg/L of sample tested, the method has a good linear relation, the limit of determination is 0.1 mg/L; at concentration levels of 0.05%, 0.50% and 5.00%, the mean recoveries are between 90.0% and 105.0% with relative standard deviations less than 5% (n=6). This method has the characteristics of high universality, good selectivity and high sensitivity, it can meet the requirements of determination of four plant growth regulators, forchlorfenuron, uniconazole, naphthylacetic acid and abscisic acid, in foliar fertilizers at the same time.

Keywordshigh performance liquid chromatographyfoliar fertilizerforchlorfenuronuniconazolenaphthylacetic acidabscisic acid

氯吡脲、萘乙酸、脱落酸和烯效唑是国内外广泛使用的4种植物生长调节剂，其中：氯吡脲对植物生长起促进作用，广泛应用于猕猴桃、葡萄、苹果、黄瓜等作物上[1]；萘乙酸可以增加坐果、防止落果和改变雌雄花比率[2]；脱落酸可以抑制种子提前萌发、诱导休眠，用于种子的储藏[3]；烯效唑作为植物生长延缓剂，广泛应用于果树、烟草和花卉等植物[4]。随着施肥技术的发展，近年来叶面施肥已成为强化作物吸收营养和防止发生某些缺素症状的有效措施，但在叶面肥生产、销售过程中非法添加农药(包括植物生长调节剂)的现状，使农业生产安全存在重大隐患[5]，建立肥料中这些物质的检测方法尤为迫切。

氯吡脲和烯效唑含量的检测多采用高效液相色谱(HPLC)法[6- 12]，萘乙酸的检测可采用化学法、气相色谱法和液相色谱法[13- 16]，而HPLC法和放射免疫法常被用于检测脱落酸[17- 18]。……