袁梦麒 潘永贵 张伟敏 李艺筱 何凤平 李尚斌



摘 要 以海南主栽品种‘中白番木瓜为试材,采用15 mmol/L甜菜碱浸泡处理,随后于冷害温度6 ℃下贮藏,研究甜菜碱处理对番木瓜冷害及其抗氧化代谢的影响。结果表明,甜菜碱处理可有效地抑制番木瓜果实SOD、POD和CAT酶活性的下降,使超氧阴离子产生速率和过氧化氢浓度保持较低的水平,减少细胞膜透性的增加和膜脂过氧化产物MDA的积累,减缓了番木瓜果实采后冷害的发生。
关键词 甜菜碱;番木瓜;冷害;抗氧化系统
中图分类号 S667.9 文献标识码 A
Abstract The papayas variety‘Zhong Baifrom Cheng-mai was used in the study. After picking, the fruit was immersed in the 15 mmol/L betaine solution and then was stored under 6 ℃ for 40 days. The change of the chilling-injury degree and anti-oxidant system were determined per 10 days. The results suggested that the betaine treatment could efficiently inhibit the decrease of the activities of various enzymes, such as SOD, POD and CAT, keep the degree of super oxygen anion producing rate and hydrogen peroxide concentration low, reduce the increase of cell membrane permeability and the accumulatation of MDA products of membrane lipid peroxidation. It can be concluded that the betaine treatment could inhibit the chilling injury of papayas after picking。
Key words Betaine; Papaya; Chilling injury; Antioxidant system
doi 10.3969/j.issn.1000-2561.2016.08.022
番木瓜(Carica papaya L.)果实具有丰富的营养价值和药用价值,近年来市场对于番木瓜的需求量逐年上升。但番木瓜属于呼吸跃变型水果,采后极易软化腐烂[1]。低温贮藏能够保持果实的采后品质,延长其货架期[2]。但是对于番木瓜这类热带亚热带水果来说,不恰当的低温会导致果实出现冷害[3]。近年来研究发现甜菜碱处理对于果蔬采后冷害有着显著的缓解作用。甜菜碱(glycine betaine,GB)属于季胺型水溶性生物碱,是植物组织细胞内的一种重要的调节物质[4]。GB的积累有助于维持细胞渗透压、保护细胞膜结构和酶活性,增强植物抗逆性等[3]。研究表明,外源甜菜碱处理桃果实可以有效地降低其细胞膜渗透性和细胞中MDA含量,有效地维持桃果实细胞膜完整性,并减轻桃果实低温贮藏过程中的冷害[5]。甜菜碱处理有助于提高植物和果实细胞的抗氧化能力。5 mmol/L甜菜碱处理辣椒果实,发现其过氧化氢酶和过氧化物酶的活性都得到提高,冷害情况也得到缓解[6]。……