冠心病气虚血瘀证大鼠模型的建立

黄烁 任建勋 王少辰 杨斌 董小霞 许晴 王琼 刘建勋

[摘要]應用疲劳跑步运动复合左冠状动脉前降支高位结扎的方法建立冠心病气虚血瘀证病证结合大鼠模型。该实验选用Wistar雄性大鼠，随机分为假手术组（JSS）、单纯结扎冠脉组（DZ）、疲劳跑步运动复合结扎冠脉组（PZ）。DZ组进行单纯冠脉结扎；PZ组利用小动物跑台进行为期2周的跑步运动造成疲劳模型，在此基础上进行冠脉结扎，术后以1次/2 d的频率维持28 d的力竭跑步；于术后28～31 d观察大鼠宏观体征，分别采集大鼠超声心动检测指标、呼吸幅度对大鼠冠心病气虚血瘀证的主症进行评价；采集空场实验相关指标、力竭运动时间、足底图像色彩饱和度对兼症进行评价；采集舌面色彩饱和度对舌象进行评价；采集脉搏搏动幅度对脉象进行评价。同时对血液流变学、凝血功能等指标进行检测。结果显示，术后第28天开始，PZ组大鼠主症、兼症、舌象、脉象均符合冠心病气虚血瘀证证候表现，结合相关病理性检测结果证明以疲劳跑步运动复合左冠状动脉前降支高位结扎的方法在大鼠体内可以成功建立冠心病气虚血瘀证病证结合模型。

[关键词]冠心病；病证结合；气虚血瘀证；大鼠；动物模型

[Abstract]To establish Qideficiency and bloodstasis syndrome type coronary heart disease models by fatigue running exercise and high ligation of left anterior descending coronary artery （LAD）， male Wistar rats were selected and randomly divided into sham operation group （JSS）， coronary ligation group （DZ）， fatigue running exercise+coronary ligation group （PZ） Coronary ligation alone was done in DZ group； while the rats in PZ group had running exercise in on the animal treadmill system for 2 weeks to establish fatigue models， and then coronary ligation was done based on the models The exhausted running was maintained for 28 days at the frequency of 1 time/2 days after operation Twentyeight to thirtyone days after the operation， all the rats were observed for macroscopic physical signs， and ultrasonic echocardiography indexes and breathing extent of the rats were collected to evaluate the main symptoms of rats with Qideficiency and bloodstasis syndrome type coronary heart disease； related indexes of open field test， exhaustive running time， and colorimetric analysis data on images of plantar were collected to evaluate the accompanied symptoms； colorimetric analysis data on lingual surface was collected to evaluate the tongue characteristics； pulse distension data was collected to evaluate the pulse condition， and meanwhile， blood rheology and coagulation function were also detected From the 28th day postoperatively， the main symptoms， accompanied symptoms， tongue characteristics and pulse conditions of rats in PZ group conformed to the symptoms of coronary heart disease and Qideficiency and bloodstasis syndrome Combined with related pathological results， the study revealed that Qideficiency and bloodstasis syndrome type coronary heart disease models could be successfully established by fatigue running exercise and high ligation of left anterior descending coronary artery for the rats

[Key words]coronary heart disease； diseasesyndrome combination； Qideficiency and bloodstasis； rat； animal model

doi：10.4268/cjcmm20160921

血管疾病是目前导致人类死亡与残疾的三大主要疾病之首，严重危害人类健康。世界卫生组织（WHO）最近的报告中指出，2000—2020年心肌梗死的死亡率将由第5位上升到第1位，因心血管病引发的死亡占全球死亡人数的30%，该病严重影响着广大患者的健康。……