灰树花多糖D组分对细颗粒物诱导的人支气管上皮细胞损伤的保护作用

茹　琴　熊　琪　田　香　陈　琳　徐丛玥　李超英，2▲.湖北省江汉大学武汉生物医学研究院，湖北武汉　430056；2.湖北省汉济生物科技（武汉）有限公司，湖北武汉　430075

灰树花多糖D组分对细颗粒物诱导的人支气管上皮细胞损伤的保护作用

茹琴1熊琪1田香1陈琳1徐丛玥1李超英1，2▲
1.湖北省江汉大学武汉生物医学研究院，湖北武汉430056；2.湖北省汉济生物科技（武汉）有限公司，湖北武汉430075

目的 研究灰树花多糖D组分（D-fraction）对细颗粒物（SRM 2786）诱导的人支气管上皮细胞（16HBE）损伤的保护作用。方法 利用细胞增殖实验检测SRM 2786或D-fraction对16HBE细胞增殖的影响，确定给药浓度；将16HBE细胞分7组，分别为对照组、模型组（125 μg/mL SRM 2786）和不同浓度D-fraction给药组（分别给予12.5、25、50、100、200 μg/mL D-fraction，同时给予125 μg/mL SRM 2786），细胞增殖实验检测细胞存活率，酶联免疫法检测细胞培养液白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和粒细胞巨噬细胞刺激因子（GM-CSF）的含量。结果31.25～500 μg/mL SRM 2786处理使细胞存活率显著降低（P＜0.05）。125 μg/mL的SRM 2786刺激使16HBE细胞IL-1β、IL-6、TNF-α和GM-CSF释放显著升高（P＜0.05），D-fraction（≥100 μg/mL）显著抑制SRM 2786诱导的炎症细胞因子释放（P＜0.01），且能显著提高损伤细胞存活率。结论 细颗粒物能抑制16HBE细胞增殖、诱导炎症因子释放，D-fraction对细颗粒物造成的细胞损伤有显著保护作用，其作用机制可能与抑制炎症因子释放有关。

细颗粒物；炎性损伤；灰树花多糖D组分；人肺支气管上皮细胞

［Abstract］Objective To investigate the protective effect of Grifola frondosa D fraction（D-fraction）on human bronchial epithelial（16HBE）cell damage that induced by fine particulate matter（SRM2786）.Methods MTT assay was used to detect the survival rate of 16HBE cells treated with SRM2786 or D-fraction，and the concentration of SRM2786 or D-fraction used for further experiments was selected.16HBE cells were divided into seven groups，including control group，model group（125 μg/mL SRM 2786），D-fraction group（125 μg/mL SRM 2786+12.5 or 25 or 50 or 100 or 200 μg/mL D-fraction）.MTT assay was used to detect the survival rate，and enzyme-linked immune kits were used to detect the release of interleukin-1β（IL-1β），interleukin-6（IL-6），tumor necrosis factor-α（TNF-α）and granulocyte-macrophage colony-stimulating factor（GM-CSF）.Results Compared with the control group，the cell survival rates of 16HBE cells treated with SRM 2786（31.25-500 μg/mL）were decreased remarkably（P＜0.05）.Treatment with 125 μg/mL SRM 2786 could significantly increase the release of IL-1β，IL-6，TNF-α and GM-CSF（P＜0.05）.D-fraction（≥100 μg/mL）could not only significantly improve the survival rate，but also significantly inhibit the release of inflammatory cytokines（P＜0.01）.Conclusion Fine particulate matter can inhibit cell proliferation and induce the release of inflammatory cytokines.D-fraction has a significant protective effect of cell damage caused by fine particulate matter，and the mechanism may be related to inhibit the release of inflammatory factors.

［Key words］Fine particulate matter；Inflammation damage；Grifola frondosa D-fraction；Human lung bronchial epithelial cells

细颗粒物（fine particlate matter，空气动力学直径≤2.5 μm的颗粒）是雾霾中的首要污染物［1］，不易被鼻腔绒毛阻挡，能抵达细支气管壁，引发哮喘、支气管炎和心血管病等疾病［2］。细颗粒物浓度每增加10 μg/m3，总死亡风险会上升4%，心肺疾病死亡风险上升6%，肺癌死亡风险上升8%［3］。……