经皮芒硝超声导入对急性胰腺炎大鼠模型的实验研究*

金佳佳 陈建华 杨伟刚（浙江省湖州市中医院，浙江 湖州 313000）

·研究报告·

经皮芒硝超声导入对急性胰腺炎大鼠模型的实验研究*

金佳佳 陈建华 杨伟刚
（浙江省湖州市中医院，浙江 湖州 313000）

目的 观察经皮芒硝超声导入对急性胰腺炎大鼠的影响。方法 取清洁级SD大鼠90只，按随机数字表法随机分成3组，空白对照组（A组）、传统芒硝外敷组（B组）、经皮芒硝超声导入组（C组）。B、C组袋中装有30 g芒硝，A组袋中装有同样重量铝片。C组大鼠运用超声不间断超声导入，每12小时更换芒硝1次。将各组大鼠血液进行血清肿瘤坏死因子-α（TNF-α）和血清淀粉酶（AMY）检测，对胰腺组织病理变化进行评分，观察各组大鼠急性胰腺炎模型胰腺损伤程度、不同时相各组大鼠胰腺组织的病理评分的比较以及各组大鼠血清TNF-α及AMY的水平。结果 从病理改变轻重程度，C组>B组>A组；B组和C组大鼠的病理评分均明显低于A组（P＜0.05）；与B组相比，C组大鼠第2日和第3日的病理学评分均更低（P＜0.05）；与A组相比，B组的血清TNF-α和AMY水平降低（P＜0.05）；C组各指标均较B组显著降低（P＜0.05）。结论 经皮芒硝超声药物导入法对急性胰腺炎大鼠模型的疗效明显优于单一传统给药疗法。

急性胰腺炎 经皮超声导入 芒硝 实验研究

【Abstract】Objective：To explore the observation of mirabilite by percutaneous ultrasound guided for rat model with acute pancreatitis.Methods：90 clean grade SD rats were selected，which were randomly divided into 3 groups：the control group（group A），the group of traditional external application of mirabilite（group B），the group by Percutaneous ultrasound guided mirabilite（group C）.Group B and group C were filled with 30 g of mirabilite，as well as group A with the same weight of aluminum sheet.The rats in group C were treated with mirabilite by percutaneous ultrasound guided，changed once every 12 hours.The blood samples were taken and serum tumor necrosis factor-α（TNF-α），amylase（AMY）in different groups were compared；simultaneously the pathological changes of pancreatic tissue were scored.Then the degree of pancreatic injury，pathological score of model rats′tissues of acute pancreatitis and the level of serum TNF-α，AMY in the three groups were observed and compared.Results：From the comparison of pathological changes of the degree，the order was group C>group B> group A；the pathological scores of group B and group C were significantly lower than that of group A（P＜0.05）；compared with group B，the pathological scores of group C on the second day and the third day were lower（P＜0.05）；the levels of serum TNF-α and AMY in group B were lower appreciably than that of group A，and the difference was statistically significant（P＜0.05）.Each index in group C was significantly lower than that of group B （P＜0.05）.Conclusion：The curative effect of percutaneous ultrasound drug delivery method of mirabilite on rat models with acute pancreatitis is better than that of the traditional single drug therapy，worthy of further clinical research and exploration henceforward.

【Key words】Acute pancreatitis；Percutaneous ultrasonic introduction；Mirabilite；Experimental study

急性胰腺炎（AP）是多种病因导致胰酶在胰腺内被激活后引起胰腺组织自身消化、水肿、出血甚至坏死的炎性反应，是临床较为严重的一种急腹症［1］。现代许多研究证明，芒硝外敷具有消炎止痛、预防感染、吸收腹腔渗液、促进脓肿吸收、促进与恢复消化道功能等功效［2］。笔者通过……