五加芪粉对鸡免疫新支流三联灭活疫苗免疫效果的影响

潘贵珍 高艳艳 李召斌等

摘要[目的]研究五加芪粉对鸡免疫新支流三联灭活疫苗免疫效果的影响。[方法]将五加芪粉与新支流三联灭活疫苗配合使用，探讨五加芪粉对鸡新城疫抗体和禽流感抗体水平以及外周血淋巴细胞增殖情况的影响。[结果]35日龄、42日龄各组鸡体内抗体水平逐渐上升，其中五加芪粉中、高剂量组与疫苗对照组差异达显著或极显著水平；28、35、42日龄，中、高剂量五加芪粉显著或极显著地促进淋巴细胞增殖。[结论]五加芪粉与鸡新支流三联灭活苗联用具有提高鸡非特异性免疫机能及疫苗免疫效果的作用。

关键词五加芪粉；新支流三联灭活疫苗；免疫效果

中图分类号S858.31文献标识码A文章编号0517-6611（2016）04-154-03

Effect of Wujiaqi Powder on Immune Efficacy of Inactivated Vaccine by NDIBAIV in Chicken

PAN Guizhen， GAO Yanyan， LI Zhaobin et al（Luoyang Huizhong Animal Medicine Co.， Ltd， National Research Center for Veterinary Medicine， Luoyang， Henan 471003）

Abstract[Objective] To explore the effect of Wujiaqi powder on immune efficacy of inactivated vaccine by NDIBAIV in chicken. [Method] By the combination use of Wujiaqi powder and new tributary inactivated vaccine， effects of Wujiaqi powder on newcastle disease antibody and avian influenza antibody level were studied， as well as peripheral blood lymphocyte proliferation. [Result] On 35 and 42 days， the groups which applied low and medium dosage Wujiaqi Powder could significantly improve the immune efficacy of newcastle disease and avian influenza. On 28， 35 ，42 days， the results showed that Wujiaqi Powder was able to stimulate proliferation of peripheral splenic lymphocytes. [Conclusion] Combination of Wujiaqi powder and new tributary inactivated vaccine could signicantly improve the immune efficacy of vaccine.

Key wordsWujiaqi powder； NDIBAI tributary inactivated vaccine； Immune effect

养鸡在我国畜禽养殖业中占很大比例，而禽病始终制约着我国养殖业的发展。新城疫和禽流感以其发病急、死亡率高而成为我国养鸡业危害最大、经济损失最重的禽病之一，并被国际兽疫局（OIE）列为A类疫病[1]。我国目前主要采取预防接种来减少该病的发病率，但在实际生产中经常由于气温、环境应激、药物和病原等多种因素的干扰，造成免疫状态低下，致使机体对外源性刺激应答不力，导致疫苗免疫效果不理想[2-3]。黄芪和刺五加具有很好的免疫调节作用，常被用于解除动物免疫抑制状态。杨一清等[4]报道黄芪刺五加合剂对免疫抑制小鼠免疫功能具有上调作用，而刺五加和黄芪具有一定的协同作用。张翠[5]报道黄芪和刺五加配伍组方后配合新城疫疫苗使用可以提高鸡新城疫病毒抗体水平，促进雏鸡淋巴细胞增殖，在一定程度上降低鸡发病率和死亡率。……