补肾健骨汤对去卵巢大鼠股骨颈骨小梁微血管和骨密度的影响

王　勇，黄永松，李冬春，胡　拥，王　威

（湖南中医药大学第二附属医院，湖南长沙410005）

补肾健骨汤对去卵巢大鼠股骨颈骨小梁微血管和骨密度的影响

王勇，黄永松，李冬春，胡拥，王威

（湖南中医药大学第二附属医院，湖南长沙410005）

目的探讨补肾健骨汤对股骨颈骨小梁微血管及骨密度影响。方法采用切除大鼠卵巢方法建立骨质疏松症模型，建模成功后随机分为正常组、假手术组、去势模型组、雷洛昔芬组、补肾健骨汤组，电镜观察股骨颈骨小梁微血管变化以及骨密度检测股骨颈骨密度。结果（1）正常组微血管规则、连续，骨小梁连续且致密，厚度均匀；去势模型组微血管变形变窄，断裂，骨小梁稀疏、萎缩，连续中断；补肾健骨汤、雷洛昔芬组骨小梁微血管粗细较均匀，骨小梁出现部分断裂、排列欠规则。（2）假手术组与正常组骨密度无差异（P＞0.05）；去势模型组骨密度下降明显，但低于补肾健骨汤组及雷洛昔芬组（P＜0.05）。结论补肾健骨汤可有效改善股骨颈骨小梁微结构并提高骨密度，说明其对绝经后骨质疏松症的防治及降低骨折发生起到积极作用。

绝经后骨质疏松症；去卵巢大鼠；骨小梁微血管；骨密度；补肾健骨汤；熟地黄；淫羊藿；骨碎补

〔Abstract〕Objective To investigate the effects of Bushen Jiangu decoction on femoral neck bone trabecular microvascular and its bone mineral density（BMD）in ovariectomized rats.M ethods The osteoporosis model rats were built by their ovaries removed，which were assigned to the normal group，sham-operation group，model group，raloxifene group，Bushen Jiangu decoction group.The changes of femoral neck bone trabecular capillaries were observed by electron microscopy and its BMD was detemined.Results（1）The microvessel of normal group was regular and continuous，bone trabecula was continuous，compact and with uniformity of thickness.The microvessel of ovariectomized models was narrow deformation，fracture，bone trabecular sparse，atrophy and break of continuous.The bone trabecular microvascular of Bushen Jiangu decoction and raloxifene groups was with uniform thickness，bone trabecular appear some fault，arranging owe rules.（2）There is no difference between control group and sham-operation group（P＞0.05）；the ovariectomized rats's BMD was reduced significantly，but it was lower than that of Bushen Jiangu decoctionand raloxifene groups（P＜0.05）.Conclusion Bushen Jiangu decoction can improve the ovariectomized rat`s femoral neck bone structure and its bone mineral density.It is proving that Bushen Jiangu decoction can cure postmenopausal osteoporosis and reuce the rate of fracture.

〔Keywords〕postmenopausal osteoporosis；ovariectomized rats；trabecular microvessel；bone mineral density；Bushen Jiangu decoction；Radix Rehmanniae Preparata；Herba Epimedii；Rhizoma Drynariae

随着老龄化社会的到来，影响妇女生活质量的绝经后骨质疏松症（postmenopausal osteoporosis，PMO）的发病率越来越高，由其引起的骨折及并发症日益受到关注。在临床应用中发现，具有补肾益骨功效的补肾健骨汤能够有效地改善妇女PMO的症状，为进一步明确研究组经验方“补肾健骨汤”作用机制，本文以去卵巢（去势）大鼠建立PMO模型，使用扫描电镜观察去势大鼠股骨颈骨小梁微血管变化，骨密度仪检测大鼠股骨颈骨密度来探讨补肾健骨汤对PMO的防治作用。……