香砂六君子汤对脾胃虚弱型萎缩性胃炎大鼠胃窦黏膜组织白细胞介素—6、10及热休克蛋白70表达的影响

王强 王庆胜 鲁鹏程 成映霞 李兰珍 段云燕 段永强 杜娟 刘雪松

摘要：目的 观察香砂六君子汤对脾胃虚弱型慢性萎缩性胃炎（CAG）大鼠胃窦黏膜组织白细胞介素（IL）-6、IL-10及热休克蛋白70（HSP70）基因及蛋白表达的影响，探讨其作用机制。方法 受试大鼠按随机数字表法分为空白组和造模组，采用综合法复制脾胃虚弱型CAG动物模型，将成模大鼠随机分为模型组、阳性对照组和香砂六君子汤高、中、低剂量组。空白组和模型组大鼠予10 mL/（kg·d）蒸馏水灌胃，香砂六君子汤高、中、低剂量组分别予24、12、6 g/（kg·d）香砂六君子汤药剂灌胃，阳性对照组予0.30 g/（kg·d）维霉素药剂灌胃，连续120 d。检测各组大鼠胃窦黏膜组织IL-6、IL-10含量，实时荧光定量PCR检测胃窦黏膜组织IL-6、IL-10和HSP70 mRNA表达，蛋白免疫印迹法检测HSP70蛋白表达。结果 与空白组比较，模型组大鼠一般生存状况较差，大鼠胃窦黏膜组织IL-6 mRNA表达、IL-6含量显著升高（P<0.05，P<0.01），IL-10 mRNA表达、IL-10含量显著降低（P<0.05，P<0.01），HSP70基因和蛋白表达显著降低（P<0.05，P<0.01）；与模型组比较，香砂六君子汤高剂量组大鼠一般生存状况明显改善，大鼠胃窦黏膜组织IL-6 mRNA表达、IL-6含量显著降低（P<0.05，P<0.01），IL-10 mRNA表达、IL-10含量显著升高（P<0.05），香砂六君子汤高、中剂量组HSP70基因和蛋白表达显著升高（P<0.05）。结论 香砂六君子汤对脾胃虚弱型CAG大鼠胃窦黏模具有保护作用。

关键词：香砂六君子汤；萎缩性胃炎；白细胞介素-6；白细胞介素-10；热休克蛋白70；大鼠

DOI：10.3969/j.issn.1005-5304.2016.11.015

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）11-0062-05

Abstract： Objective To observe the effects of Xiangsha Liujunzi Decoction （XSLJZ） on levels and mRNA of IL-6， IL-10 and protein expression of HSP70 of gastric mucosa in chronic atrophic gastritis （CAG） rats with spleen-stomach deficiency； To discuss its mechanism. Methods Rats were divided into 2 groups through random number table： normal group and model group. The model of CAG rat with spleen-stomach deficiency type was induced by synthetic methods. After successful modeling， rats were divided into model group， positive control group， XSLJZ high-， medium-， low-dose group. Rats in normal and model group received distilled water 10 mL/（kg·d） for gavage； XSLJZ high-， medium-， low-dose group received XSLJZ 24， 12， 6 g/（kg·d）， respectively； positive control group received mycin 0.30 g/（kg·d） for gavage for 120 consecutive days. Generally living conditions， levels and mRNA of IL-6， IL-10 and protein expression of HSP70 in gastric mucosa tissue were detected by protein immunoblotting. Results Compared with the normal group， generally living conditions of rats in the model group were poor； mRNA and the content of IL-6 increased significantly， and mRNA and the content of IL-10 decreased significantly （P<0.05， P<0.01）； mRNA and protein expression of HSP70 in gastric tissues was much lower than that of normal group （P<0.05， P<0.01）. Compared with model group， generally living conditions of rats in the XSLJZ high-dose groups were improved significantly； mRNA and the content of IL-6 decreased significantly， and mRNA and the content of IL-10 increased significantly （P<0.05）； XSLJZ high-， medium-dose groups mRNA and protein expression of HSP70 in gastric tissues increased significantly （P<0.05）. Conclusion XSLJZ has protective effects on gastric mucosa of CAG rats with spleen-stomach deficiency.

Key words： Xiangsha Liujunzi Decoction； atrophic gastritis； IL-6； IL-10； HSP70； rats

慢性萎缩性胃炎（chronic atrophic gastritis，CAG）是消化系统多发疾病，病理表现为胃黏膜固有腺体萎缩、腺体细胞数量减少且伴有功能丧失，严重者伴有慢性持续性炎性反应以及肠上皮化生和不典型增生。中医认为，CAG多以脾胃虚弱，气机（或气血）失调为主要病机，故治疗多以健脾益气、理气和胃为大法。香砂六君子汤出自《古今名医方论》，是防治脾胃虚弱型CAG的有效复方。临床研究表明，运用香砂六君子汤及其加减方治疗CAG疗效显著[1-2]。本实验观察香砂六君子汤对脾胃虚弱型CAG大鼠一般生存状况，胃窦黏膜组织白细胞介素（IL）-6、IL-10分泌水平，热休克蛋白70（HSP70）基因及蛋白表达的影响，探讨该复方防治CAG的作用机制。……