冰冻红细胞的制备与应用分析

黄飞??谭均强??高炳谏

[摘要] 目的 研究并探讨冰冻红细胞的制备方法及应用价值。 方法 对100份悬浮红细胞（2016年6月～2017年1月本站采集2～6d内的RH（D）阴性红细胞16U）标本在不同条件下进行制备，制备方法为甘油化、去甘油化，分别在不同放置时间、不同离心速度下将其制备成冰冻红细胞，比较加甘油后不同放置时间的甘油残留量、去甘油化过程中不同离心速度的各项冰冻红细胞参数。 结果 加甘油后不同放置时间下，放置30min的标本甘油残留量明显低于放置15min的标本（P<0.05）；不同离心速度下，去甘油化后冰冻解冻红细胞的各项参数均符合国家质检标准，离心速度2600转/min的标本其甘油残留量、体外溶血率、游离血红蛋白均明显低于离心速度3300转/min的标本（P<0.05），且其红细胞回收率明显高于离心速度3300转/min的标本（P<0.05）。 结论 对红细胞标本进行甘油化、去甘油化制备，可获得优质冰冻红细胞，有利于保证输血的安全性，缓解血液供需矛盾。

[关键词] 冰冻红细胞；制备；去甘油化；输血

[中图分类号] R457.1 [文献标识码] A [文章编号] 2095-0616（2017）11-146-03

Preparation and application of frozen red blood cells

HUANG Fei TAN Junqiang GAO Bingjian

Yunfu Central Blood Station，Yunfu 445300，China

[Abstract] Objective To study and explore the preparation method and application value of frozen red blood cells. Methods 100 suspension with white and red cell specimens （RH（D） negative erythrocyte 2-6d（16U） were collected in this station from June 2016 to January 2017） were prepared under different conditions.The frozen red blood cells were preparated by the preparation method of glycerol and glycerol，respectively in different time and different centrifugal speed under，at different times after the addition of glycerol for glycerol residues，red blood cell the parameters of different centrifugal speed in the process of freezing glycerol. Results The time of different placement with glycerol，glycerol residues placed specimens of 30min were significantly lower than that of 15min（P<0.05） were placed.Different centrifugal speeds，deglycerolizing after the parameters of frozen thawing red blood cell were in line with national quality inspection standards，centrifugal speed 2600rpm /min were the glycerol residue and residual white blood cells in vitro，and free hemoglobin were significantly lower than that of centrifugal speed 3300rpm/min samples （P<0.05），and the recovery rate of RBC was significantly higher than that of centrifugal speed 3300rpm/min samples （P<0.05）. Conclusion The red blood cells can be obtained by high quality frozen red blood cells.It is helpful to ensure the safety of blood transfusion and relieve the contradiction between supply and demand of blood.

[Key words] Frozen red blood cells；Preparation；De glycerol；Blood transfusion

冰凍红细胞是利用冰冻保护剂将红细胞保存于低温环境下，冰冻红细胞的保存时间长，且能够维持红细胞原有的ATP及2，3-dpg水平，可有效解决患者急救输血，同时，自身输血还可防止输血反应与输血相关疾病的传播。但值得注意的是，红细胞的代谢速度受保存温度的影响。冰冻红细胞是缓解血源供需矛盾、应对突发事件时采取的重要措施，而关于如何制备冰冻红细胞关系到冰冻解冻红细胞的质量[1-2]，本次研究采用ACP125全自动血细胞处理仪对冰冻红细胞进行甘油化以及去甘油化制备，并对其冰冻解冻红细胞质量进行分析后发现，各项参数均符合质量标准，现就研究进行报道。

1 资料与方法

1.1 一般资料

悬浮红细胞标本共100袋（每袋2U），北京博德桑特输采血器材科技开发中心生产的57%甘油160mL、9%氯化钠溶液80mL，山东威高集团医用高分子……