苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量Δ

白瑞斌，马玉玲，张 培，王燕萍，李应东，胡芳弟#（.兰州大学药学院，兰州 730000；.甘肃中医学院附属医院，兰州 730000）

苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量Δ

白瑞斌1＊，马玉玲1，张 培1，王燕萍1，李应东2，胡芳弟1#（1.兰州大学药学院，兰州 730000；2.甘肃中医学院附属医院，兰州 730000）

目的：建立一种基于苯酚-硫酸法结合校正因子法测定含半乳糖醛酸（GalA）的多糖中糖含量的方法。方法：优化苯酚-硫酸法的测定条件，以不同质量比的GalA-葡萄糖（Glc）混合对照品溶液绘制系列标准曲线并测定含GalA的党参多糖CPP1b样品中的糖含量，在此基础上，根据GalA-Glc质量比为0-100%时的回归方程，以党参多糖CPP1b为参照多糖计算校正因子，对样品中的糖含量测定结果进行校正，并以与党参多糖CPP1b组成相同的混合单糖对照品对此方法合理性进行验证。结果：经计算，党参多糖CPP1b对Glc的校正因子为3.33，验证试验中与党参多糖CPP1b组成相同的混合单糖对照品的糖含量测定结果为103.47%。精密度、稳定性试验的RSD＜1%；加样回收率为93.52%～107.35%（RSD＝5.09%，n＝6）。结论：该研究建立的方法可准确测定类似于党参多糖CPP1b的含GalA的多糖中的糖含量。

多糖；半乳糖醛酸；党参多糖CPP1b；苯酚-硫酸法；校正因子法；糖含量；测定

ABSTRACTOBJECTIVE：To establish a method for the content determination of saccharide in polysaccharides containing galacturonic acid based on phenol-sulfuric acid method combined with correction factor method.METHODS：The determination condition of phenol-sulfuric acid was optimized.A series of standard curves were drawn with glacturonic aid-glucose mixed control with different mass ratio.The content of saccharide in Codonopsis pilosula polysaccharides CPP1b samples containing galacturonic acid was determined.According to regression equation of galacturonic acid-glucose ratio of 0-100%，the correction factor was calculated by using C.pilosula polysaccharides CPP1b as the reference polysaccharide，and the results of content determination of saccharide were corrected.The rationality of this method was verified by using mixed monosaccharide control with same composition as C.pilosula polysaccharides CPP1b.RESULTS：The correction factor of C.pilosula polysaccharide CPP1b to glucose was 3.33；in validation test，the content of saccharide in mixed monosaccharide control with same composition as C.pilosula polysaccharides CPP1b was 103.47%.RSDs of precision and stability tests was＜1%.The recoveries ranged 93.52%-107.35%（RSD＝5.09%，n＝6）.CONCLUSIONS：The established method can accurately determine the content of saccharide in C.pilosula polysaccharides CPP1b containing galacturonic acid.

KEYWORDSPolysaccharides；Galacturonic acid；Codonopsis pilosula polysaccharides CPP1b；Phenol-sulfuric acid method；Correction factor method；Saccharide content；Determination

现代药理学研究证实，党参具有抗肿瘤[1]、增强机体免疫力[2]等作用，而党参多糖为其主要活性成分之一[3]。CPP1b是本课题组从党参中分离的一种酸性果胶多糖[4]，是由鼠李糖（Rha）、阿拉伯糖（Ara）、半乳糖（Gal）和半乳糖醛酸（GalA）组成的杂多糖，其中含有大量的GalA。本课题组发现，对于党参多糖CPP1b这样一个高纯度多糖，采用苯酚-硫酸法，以葡萄糖（Glc）对照品溶液制备标准曲线，所测得的糖含量仅为30.96%。……