肿瘤转移相关基因1和血管内皮生长因子在卵巢癌中表达的临床意义

郭玲　李莲娣　周淑芬

[摘要] 目的 探讨肿瘤转移相关基因1和血管内皮生长因子在卵巢癌中表达的临床意义。 方法 研究收集我院病理科存档的原发性卵巢癌标本68例为卵巢癌组，另选取同期术后切除病理确诊良性的卵巢组织30例为对照组，采用EnVision法检测MTA1以及VEGF蛋白在卵巢癌和正常卵巢组织中的表达情况以及两者的相关性。结果 MTA1蛋白在卵巢癌组中表达的例数显著高于对照组（χ2=16.596，P=0.000），VEGF蛋白在卵巢癌组中表达的例数显著高于对照组（χ2=11.049，P=0.000）；MTA1的蛋白表达与患者年龄、肿瘤分级等因素无关（P>0.05），与临床分级、淋巴结转移具有一定相关性（P<0.05）；VEGF的蛋白表达与患者年龄无关（P>0.05），与临床分期、淋巴結转移、肿瘤分级等具有一定相关性（P<0.05）；68例卵巢癌患者中的MTA1和VEGF蛋白共同表达阳性者30例，二者呈正相关（r=0.406，P<0.05）。 结论 MTA1和VEGF蛋白表达在卵巢癌的发展中均有一定的作用，二者呈协同作用，若二者水平提高则是患者预后较差的标志。

[关键词] 肿瘤转移相关基因1；血管内皮生长因子；卵巢癌；蛋白；淋巴结转移

[中图分类号] R737.31 [文献标识码] A [文章编号] 1673-9701（2017）24-0008-03

[Abstract] Objective To investigate the clinical significance of the expression of tumor metastasis-associated gene 1 and vascular endothelial growth factor in ovarian cancer. Methods 68 cases of primary ovarian cancer specimens which were archived in the department of pathology in our hospital were collected as ovarian cancer group. 30 cases benign ovarian tissues with pathological diagnosis after resection during the same period were selected as the control group. The expression of MTA1 and VEGF protein in ovarian cancer and normal ovarian tissues were detected by EnVision method and the correlation between them was detected. Results The case number of MTA1 protein expressed in ovarian cancer group was significantly higher than that in the control group（χ2=16.596，P=0.000）. The case number of VEGF protein expressed in ovarian cancer group was significantly higher than that in the control group（χ2=11.049，P=0.000）； the expression of MTA1 protein was not correlated with age， tumor grade and other factors（P>0.05）， and had a certain relationship with clinical grade and lymph node metastasis（P<0.05）； the expression of VEGF protein was not correlated with the age of the patients（P>0.05）， and it was correlated with clinical stage， lymph node metastasis and tumor grade（P<0.05）； among 68 cases of ovarian cancer patients， there were 30 patients with co-expressed MTA1 and VEGF protein， and the two were positively correlated（r=0.406，P<0.05）. Conclusion The expression of MTA1 and VEGF protein plays an important role in the development of ovarian cancer. The two have synergistic effect. If the levels of the two protein are increased， it indicates that the patients have poor prognosis.

[Key words] Tumor metastasis-associated gene 1；Vascular endothelial growth factor；Ovarian cancer；Protein；Lymphatic metastasis

相关资料显示[1，2]：卵巢癌是女性生殖器官常见的恶性疾病，发病率位于第三位，仅低于宫颈癌和子宫体癌。肿瘤转移相关基因1（MTA1）在胰腺癌、乳腺癌、前列腺癌等恶性肿瘤疾病中一般均呈现高表达[3]，并且与恶性疾病的发展进程呈相关性[4]。血管内皮生长因子（VEGF）在肿瘤进展中具有重要作用[5]。本研究收集我院病理科存档的原发性卵巢癌标本68例，与良性卵巢组织比较，探讨MTA1以及VEGF蛋白在卵巢癌和正常卵巢组织中的表达情况。现报道如下。endprint

1 资料与方法

1.1 一般资料

本研究收集2013年1月～2016年1月间我院病理科68例原发卵巢癌标本作为卵巢癌组，对入组标本重新切片，浆液性乳头状囊腺癌41例，黏液性囊腺癌12例，子宫内膜样癌15例。病理……