健脾消癌方对转化生长因子—β1诱导的SW620细胞上皮间质转化的影响

罗吉+李勇敏+简小兰+罗燕+谭小宁+蒋益兰

摘要：目的 观察健脾消癌方对转化生长因子-β1（TGF-β1）诱导的SW620细胞上皮间质转化的影响，探讨其防治结直肠癌的可能机制。方法 体外培养结直肠癌细胞SW620。CCK-8法和Transwell小室实验测定细胞增殖、侵袭及迁移能力，qRT-PCR和Western blot检测E-cadherin和Vimentin的基因和蛋白表达。结果 健脾消癌方药物血清能抑制SW620细胞体外增殖、迁移和侵袭能力；与空白组比较，TGF-β1诱导组细胞E-cadherin表达水平下降，Vimentin表达水平升高（P<0.05）；与TGF-β1诱导组比较，健脾消癌方+TGF-β1组细胞E-cadherin表达水平升高，Vimentin表达水平下降（P<0.05）。结论 健脾消癌方可通过调控TGF-β1诱导的SW620细胞上皮间质转化过程，抑制结肠癌的生长、侵袭和迁移能力，达到防止结直肠癌复发和转移的目的。

关键词：健脾消癌方；结直肠癌；肿瘤转移；上皮间质转化

DOI：10.3969/j.issn.1005-5304.2018.02.010

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）02-0042-05

Effects of Jianpi Xiaoai Prescription on Epithelial-mesenchymal Transition of

Colorectal Cancer Cell SW620 Induced by TGF-β1

LUO Ji1， LI Yong-min1， JIAN Xiao-lan2， LUO Yan1， TAN Xiao-ning1， JIANG Yi-lan1

1. Affiliated Hospital of Hunan Academy of Chinese Medicine， Changsha 410006， China；

2. Graduate School of Hunan University of Chinese Medicine， Changsha 410208， China

Abstract： Objective To observe the effects of Jianpi Xiaoai Prescription on epithelial-mesenchymal transition of colorectal cancer cell SW620 induced by TGF-β1； To discuss its possible mechanism of action for prevention and treatment of colorectal cancer. Methods Colorectal cancer cells SW620 were cultured in vitro. By conducting CCK-8 and Transwell experiments， the proliferation， invasion and migration of colorectal cancer cell （SW620） were detected. qRT-PCR and Western blot experiments were applied to verify the mRNA and protein expression level of E-cadherin and Vimentin. Results Jianpi Xiaoai Prescription showed in vitro inhibitory effect on the proliferation， invasion and migration of colorectal cancer cell SW620. Compared with blank group， the expression of E-cadherin in TGF-β1 induced group was reduced and Vimentin was increased （P<0.05）； Meanwhile， the expression of E-cadherin in Jianpi Xiaoai Prescription group was increased and Vimentin was decreased when compared with TGF-β1 induced group （P<0.05）. Conclusion Jianpi Xiaoai Prescription can inhibit the proliferation， invasion and migration of colorectal cancer cell by reverting the epithelial-mesenchymal transition of colorectal cancer cell induced by TGF-β1， with a purpose to achieve the goal of preventing and treating the recurring and migration of colorectal cancer.

Keywords： Jianpi Xiaoai Prescription； colorectal cancer； metastasis； epithelial-mesenchymal transition

结直肠癌（colorectal cancer，CRC）是临床常见的恶性肿瘤之一，发病率和病死率位居消化道恶性肿瘤的第2位[1]。本课题组既往研究表明，健脾消癌方配合化疗能显著降低大肠癌的复发与转移，控制瘤体的生长，延长患者生存期，提高患者生存质量；能抑制结直肠癌裸鼠模型的肝转移率，但其具体作用机制还不甚清楚[2-4]。上皮间质转换（epithlial-mesenchymal transition，EMT）是指上皮细胞在特定的生理和病理情况下向间质细胞转化的现象，被公认为影响CRC侵袭和转移的重要作用机制之一。在EMT过程中，上皮细胞标志蛋白E-cadeherin表达下调及间质细胞标志蛋白Vimentin表达上調，从而导致细胞极性发生改变、细胞骨架被重建、细胞间黏附出现不同程度的丧失以及肿瘤基底膜和细胞外基质均被不同程度破坏，从而使细胞获得高迁移、高侵袭、抗凋亡及降解细胞外基质等间质表型特征[5]。本实验采用转化生长因子-β1（TGF-β1）诱导的CRC细胞SW620发生EMT，建立大鼠细胞EMT模型，探究健脾消癌方对SW620细胞EMT的作用及其抗肿瘤细胞迁移侵袭的可能机制。……