云南10种花的抗肿瘤活性研究

倪广惠 赵正波 陈左丽

【摘 要】 目的：探索云南花朵的抗肿瘤活性。方法：花朵烘干后磨成粉末，用乙醇提取后浓缩得花朵提取物。将体外培养的肿瘤细胞分成对照组和试验组，每组设6个复孔。用MTS法测试花朵提取物对乳腺癌MDA-MB-231细胞的抑制作用。将有对乳腺癌MDA-MB-231细胞增殖有较好抑制作用的花朵提取物进一步进行肿瘤活性测试。将该花朵提取物分为不同浓度，测试其对乳腺癌MDA-MB-231细胞和肺癌A549细胞的抑制活性。结果：在采集来的10种花朵中，葱莲对乳腺癌MDA-MB-231细胞具有较好的抑制活性，抑制率（43.07±4.52%）与阳性对照药物（47.62±3.24%）接近。进一步研究发现，葱莲对乳腺癌MDA-MB-231细胞和肺癌A549细胞均有抑制活性，并且呈剂量效应关系。结论：葱莲花朵提取物具有较好的抗肿瘤应用前景。

【关键词】 乳腺癌；肺癌；花提取物；体外抑制；葱莲

【中图分类号】R285.5 【文献标志码】 A 【文章编号】1007-8517（2018）09-0024-03

Abstract： ObjectiveTo investigate the effects of ten flowers in Yunnan province on cancer cells. Methods Flowers were dried， pulverized and extracted with ethanol. The cancer cell proliferation inhibition of the extracts of flowers was tested by MTS assays. Human cancer cells were cultured in vitro and divided into control and test groups. Every group was set 6 wells. The positive control group was treated with 5mg/L of arsenic trioxide. The negative control group was treated 1‰ of DMSO. Test groups were treated with 20 mg/L of the extracts of flowers. The extract which showed excellent inhibition of breast cancer MDA-MB-23 cells proliferation was further tested several concentrations on breast cancer MDA-MB-23 cells and lung cancer A549 cells. Results Extract of Zephyranthes candida（Lindl.）Herb showed excellent inhibition of breast cancer MDA-MB-23 cells proliferation the concentration of 20 mg/L. The inhibition rate （43.07±4.52%）was appreciated to that of the positive control group（47.62±3.24%）. The further investigation shows that the extract inhibited cell proliferation of breast cancer MDA-MB-23 cells and lung cancer A549 cells in a dose-dependent manner. Conclusion The extract of Zephyranthes candida（Lindl.）Herb showed an excellent profile in anti-tumor.

Keywords：Breast cancer； Lung cancer； Extract of flowers； Inhition in vitro；Zephyranthes candida（Lindl.）Herb.

在《本草纲目》中，花类药物至少在80种以上，约占植物类药物的1/10以上[1]。花类质地轻灵[2]，花类药物药性较为平和，副作用小，是各名家的常用药物[3-11]，在妇科疾病中应用甚广。恶性肿瘤是严重威胁人类健康和生命的疾病，抗肿瘤药物长春花碱由夹竹桃科植物长春花中提取而来。云南鲜花四季盛开，花朵品质高、产量大[12]。笔者对云南常见的花朵进行抗肿瘤活性筛选，为进一步开发利用“云花”、“云药”提供理论依据。

1 材料与方法

1.1 细胞、试剂和仪器 乳腺癌MDA-MB-231细胞和肺癌A549细胞购自中国科学院昆明动物研究所。所有花朵采集自云南中医学院呈贡校区和昆明医科大学呈贡校区。……