咖啡酸对2型糖尿病大鼠血糖浓度的影响

裴瑜 董旭 唐心一

[摘要]目的 探討咖啡酸对2型糖尿病大鼠血糖浓度的影响。方法 选取60只6～8周龄雄性SD大鼠，适应性饲养3 d后，随机取20只大鼠作为正常观察组，分为空白组和实验组1～3，每组5只。实验组1～3分别以0.4、0.6、0.8 g/kg咖啡酸灌胃，空白组以1 ml/100 g等量蒸馏水灌胃。其余40只大鼠高糖高脂饲料连续喂养6周，连续3 d腹腔内注射30 mg/kg的链脲佐菌素（STZ），用于2型糖尿病大鼠模型的建立，FPG>7 mmol/L及大鼠尿糖实验检测阳性为造模成功。将造模成功的30只大鼠随机分为模型组、阿卡波糖组、联合组及咖啡酸组1～3，每组5只。阿卡波糖组予以10 mg/kg的阿卡波糖灌胃，联合组采用10 mg/kg的阿卡波糖以及1 g/kg的咖啡酸联合灌胃，咖啡酸组1～3分别采用0.8、1.0、1.2 g/kg咖啡酸灌胃，模型组以1 ml/100 g等量蒸馏水灌胃。观察不同时间咖啡酸对正常观察组大鼠空腹血糖浓度（FPG）及胰岛素（INS）的影响；观察各药物组对2型糖尿病大鼠FPG浓度的影响。结果 灌胃前，灌胃第1、6天，各组（正常观察组中）大鼠的FPG浓度比较，差异均无统计学意义（P>0.05）。灌胃前，各组（模型组和各药物组）的FPG浓度比较，差异均无统计学意义（P>0.05）；灌胃后，与模型组比较，各药物组大鼠的FPG浓度均显著降低，差异有统计学意义（P<0.05，P<0.01），联合组的FPG浓度降低最明显，差异有统计学意义（P<0.01）；各药物组中，以联合组的降糖效果最明显（P<0.05）。结论 咖啡酸对2型糖尿病大鼠具有降FPG作用，但不如阿卡波糖的降糖效果显著。阿卡波糖联合咖啡酸的降糖效果最明显。

[关键词]咖啡酸；阿卡波糖；2型糖尿病；降血糖

[中图分类号] R587.1 [文献标识码] A [文章编号] 1674-4721（2018）9（a）-0016-04

[Abstract] Objective To explore the influence of Caffeic Acid on blood glucose concentration in rats with type 2 diabetes mellitus. Methods Sixty male SD rats aged 6-8 weeks were selected and fed for 3 days. Twenty rats were selected as the normal observation group and they were divided into the blank group and experimental group1-3， and there were 5 rats in each group. The experimental group 1～3 was fed with 0.4， 0.6 and 0.8 g/kg of Caffeic Acid respectively， the blank group was gavaged with 1 ml/100 g of equivalent distilled water. The other 40 rats were fed with high-sugar and high-fat diet for 6 weeks and intraperitoneally injected with 30 mg/kg Streptozotocin （STZ） for 3 days， the model of type 2 diabetic rats was established， FPG>7 mmol/L and rat urine sugar test positive were considered to be successful in modeling. Thirty rats successfully modeled were randomly divided into model group， Acarbose group， combined group and Caffeic Acid group 1-3， and there were 5 rats in each group. Acarbose group was given 10 mg/kg Acarbose， combined group was given 10 mg/kg of Acarbose and 1 g/kg of Caffeic Acid， Caffeic Acid group 1-3 was given 0.8， 1.0， 1.2 g/kg respectively， model group was given 1 ml/100 g of distilled water. The influence of Caffeic Acid on fasting blood glucose （FPG） and insulin （INS） in rats of normal observation group was observed at different times； the influence of each drug group on the concentration of FPG in type 2 diabetic rats was observed. Results There was no significant difference in FPG concentration and INS expression level among the groups （in normal observation group） before gavage， gavage for the first， sixth day （P>0.05）. There was no significant difference in FPG concentration among the groups （in model group and various drug group） before gavage （P>0.05）. After gavage， compared with model group， the FPG concentration in various drug group were decreased significantly， and the differences were statistically significant （P<0.05， P<0.01）. The FPG concentration in combined group was significantly lower， and the difference was statistically significant （P<0.01）. Among the drug groups， the combined group had the most significant hypoglycemic effect （P<0.05）. Conclusion Caffeic Acid has a reducing FPG effect on type 2 diabetic rats， but it is not as effective as Acarbose in reducing blood sugar. The effect of Acarbose combined with Caffeic Acid was the most obvious.

[Key words] Caffeic Acid； Acarbose； Type 2 diabetes mellitus； Reducing the blood glucose

2型糖尿病是胰岛素分泌量相对不足或敏感性降低引发的一类代谢紊乱综合征，患者长期血糖、血脂等代谢异常，可累及多器官及系统，危害极大，应尽早治疗，并严格控制患者血糖水平[1-2]。目前主要采用药物治疗，但在治疗过程中都存在诸多副作用。阿卡波……