不同处理方式对土壤酶活性的影响

刘宇彤　霍璐阳　李志国　刘晴　董爱荣

摘要：本文分别采用靛酚蓝比色法、3.5-二硝基水杨酸法、高锰酸钾滴定的方法，探究在土壤中种植大豆、加入粘质沙雷氏菌和咪唑乙烟酸及三者相互作用对土壤脲酶、蔗糖酶、过氧化氢酶活性的影响。结果表明：在土壤中种植大豆后，土壤脲酶、蔗糖酶和过氧化氢酶活性在培养10～30 d中均呈现激活趋势;在土壤中加入粘质沙雷氏菌，5～20 d中，土壤脲酶、蔗糖酶活性均呈现激活趋势，粘质沙雷氏菌使土壤过氧化氢酶活性显著增加;在土壤中加入100 mg/kg浓度的咪唑乙烟酸，土壤脲酶呈现恢复—抑制的趋势，土壤蔗糖酶活性在10 d时开始激活，30 d时呈现抑制状态，过氧化氢酶活性在10 d和30 d时均有激活趋势;在土壤中种植大豆、加入粘质沙雷氏菌，再加入100 mg/kg的咪唑乙烟酸，土壤脲酶呈现抑制—恢复—抑制的趋势，土壤蔗糖酶活性呈现激活—恢复—抑制—激活的趋势，土壤过氧化氢酶活性呈现抑制—激活—抑制的趋势。综上所述，说明不同处理方式对土壤脲酶、蔗糖酶和过氧化氢酶活性产生的变化显著。

关键词：大豆; 粘质沙雷氏菌; 咪唑乙烟酸; 脲酶; 蔗糖酶; 过氧化氢酶

中圖分类号：S158.4文献标识码：A文章编号：1006-8023（2019）02-0021-06

Effects of Different Treatments on Soil Enzyme Activity

LIU Yutong， HUO Luyang， LI Zhiguo， LIU Qing， DONG Airong

（College of Forestry， Northeast Forestry University， Harbin 150040）

Abstract：In order to explore the effects of adding soybean， serratia marcescens and imidazolidinic acid on the soil urease， sucrase and catalase activities， the indophenol blue colorimetric method and 3.5-dinitrogen were used respectively. The results showed that the soil urease， sucrase and catalase activities showed activating trend in the culture for 10 ～ 30 d after planting soybean in the soil. Serratia marcescens was added to the soil， after 5 ～ 20 d， soil urease and sucrase activities showed activating trend， which meant that serratia marcescens significantly increased soil catalase activity. Adding 100 mg/kg imidazolidinic acid to soil， soil urease showed recovery-suppression trend， soil sucrose enzyme activity began to activate at 10 d， and inhibited at 30 d. Catalase activity was activated at 10 d and 30 d. Soy was planted in soil， added with serratia marcescens and 100 mg/kg imidazolium acid， soil urease showed a trend of inhibition-recovery-inhibition， the soil invertase activity showed a trend of activation-recovery-inhibition-activation， and soil catalase activity showed a trend of inhibition-activation-suppression. In summary， the changes in soil urease， sucrase and catalase activities were significantly different for different treatments.

Keywords：Serratia marcescens; imidazolium; urease; sucrase; catalase

0引言

化学农药多是有害物质，长期持续使用化学农药，会对生物和环境造成危害，而且由于耐药性的产生，防治效果逐渐减弱，农药的残留也给生态环境带来了许多负面影响。咪唑乙烟酸是咪唑啉酮类除草剂，由于其持效期长，所以在土壤中的残留会对下茬敏感作物产生药害，严重影响种植业结构的调整。……