龙眼Myb-related-R1的克隆及生物信息学分析

李浩然 徐炯志 潘介春 王颖 李琳 王金英 黄幸 谭春露 邓英毅

摘 要：目的：以龙眼成熟叶片为试验材料，研究Myb-related基因在龙眼中的表达情况，初步推出侧该基因的功能。方法：通过转录组测序及生物信息学分析得到龙眼Myb-related R1基因全长序列，进一步设计特异引物对其开放阅读框（ORF）全长序列进行克隆和生物信息学分析。结果：Myb-related-R1基因与SANT超家族基因片段有相似之处，ORF长度为879bp，编码292个氨基酸，有1个Myb结合位点。该蛋白属于非跨膜亲水蛋白，亚细胞定位预测定位于细胞核，存在35个氨基酸磷酸化位点。其二级结构主要由α-螺旋和无规则卷曲构成，二级结构与三级结构预测结果高度一致，同源基因进化树分析表明，该基因与阿月浑子蛋白组亲缘关系最近。结论：根据其他物种该基因的功能及生物信息学软件预测结果，可初步推测该基因功能与染色体端粒结构有关，且与花青素合成有关。

关键词：龙眼;基因克隆;Myb-related-R1;端粒

中图分类号 S667.2文献标识码 A文章编号 1007-7731（2020）09-0012-06

Cloning and Bioinformatics Analysis of Myb-related-R1 in Longan

Li Haoran et al.

（1Collegeof Agriculture，Guangxi University，Nanning 530004， China）

Abstract： Objective： the Myb-related gene family has different functions in plants.In this study， the expression of this gene in the mature leaves of longan was studied， and the function of this gene was derived.Methods： the full-length sequence of longan Myb-related-R1 gene was obtained by transcriptome sequencing and bioinformatics analysis， and further specific primers were designed for cloning and bioinformatics analysis of the full-length sequence of open reading frame （ORF）.Results： the Myb-related-R1 gene is similar to the SANT superfamily gene fragment.The length of ORF is 879bp， encoding 292 amino acids， and there is a Myb binding site.This protein belongs to non-transmembrane hydrophilic protein， which is located in the nucleus by subcellular localization prediction and has 35 amino acid phosphorylation sites.The secondary structure was mainly composed of ml-helix and irregular curl， and the predicted results of the secondary structure and tertiary structure were highly consistent.Conclusion： according to the function of this gene in other species and the prediction results of bioinformatics software， it can be preliminarily speculated that the function of this gene is related to telomeres and anthocyanin synthesis.

Key words： Longan;Gene cloning ; Myb-related-R1;Telomeres

龙眼（Dimocarpus longan Lour.）是我国南方果树，俗称桂圆，是广西地区主要栽培的果树种类之一[1-2]。因品种结构不合理，导致国内龙眼產期集中、大小年明显等问题凸显，使我国龙眼产业在国际竞争中处于劣势。为了有效调节龙眼产期，提高生产效益，许多学者开展了龙眼成花机理、产期调节技术研究及其优良品种的选育[3]，对龙眼成花分子生物学的研究逐步成为人们关注的重点。

目前，已有大量的Myb-related基因从植物中分离出来，但只有少数基因的功能为人所知。……