符洪宇 杨明 黄明亚 熊欣竹 李维



摘 要:利用水提醇沉法对香椿子多糖的提取工艺进行了优化。通过单因素和正交试验,对提取香椿子多糖的工艺进行了研究。结果表明:影响香椿子多糖最终提取量的因素主次排序:提取次数>提取时间>料液比>提取温度;最佳提取工艺:料液比1∶18,提取时间2.5h,提取温度85℃,提取次数3次。在此工艺条件下,香椿子多糖提取量达到23.77mg/g。
关键词:香椿子多糖;水提醇沉法;提取;工艺优化
中图分类号 S644.4;O629.12文献标识码 A文章编号 1007-7731(2020)09-0127-04
Extraction Technology of Toona Sinensis Polysaccharides
Fu Hongyu1 et al.
(1Food College, Sichuan Tourism University, Chengdu 610100, China)
Abstract: In this experiment, the extraction process of polysaccharides from Toon sinensis was optimized by water extraction and ethanol precipitation. The process of extracting polysaccharides from Toona sinensis was studied by single factor and orthogonal experiments. The experimental results showed that the main factors affecting the final extraction amount of polysaccharides from the seeds were: extraction times>extraction time>material-liquid ratio>extraction temperature. The rental price extraction process was as follows: the ratio of material to liquid is 1∶18, the extraction time was 2.5h, the extraction temperature was 85°C, and the extraction was performed 3 times. Under this process condition, the extraction amount of the polysaccharide from Toon sinensis was 23.77mg/g.
Key words: Polysaccharide from Toon sinensis; Cater Extraction Alcohol Precipitation; Extraction; Process Optimization
香椿廣泛分布于我国陕西、四川、安徽以及长江南北区域,具有药用价值、食用价值和观赏价值。香椿子自汉代以来就应用于中医领域,作为一种中药材使用。香椿子辛、苦、温、祛风、散寒、止痛。香椿子多糖含量较高,目前有学者对其相关性质进行了研究,丁世洪等采用超声波辅助提取香椿子多糖,采用L9(34)正交试验设计优选提取工艺,最终确定香椿子多糖水提工艺优化条件为液料比1∶12,提取120min,提取3次时提取率最高,并对香椿子多糖体外抗氧化性的研究,香椿子多糖具有一定的抗氧化活性,对DPPH自由基(DPPH·)具有较好清除能力[1]。鄢文霞等研究了香椿子多糖对小鼠和高凝模型大鼠的抗凝血作用,发现香椿子多糖对小鼠和高凝模型大鼠具有抗凝血作用,其抗凝机制可能与抑制内外源性凝血途径、升高ATIII活性、提高纤溶活性和抑制血小板凝聚有关[2]。香椿子分布广泛,用途除了作为中药材之外少之又少,利用率低,因此对香椿子多糖的提取工艺进行研究,以高效安全节能的工艺方法提取香椿子多糖,才能进行工业化提取、多维度的研究,广泛地应用到各个领域。……p>