柴胡皂苷d抗肝纤维化模型中的大鼠脂质过氧化作用分析

金红阳 张坤 胡海 孙洁

【摘要】目的：分析柴胡皂苷 d 抗肝纤维化模型中的大鼠脂质过氧化作用。方法：将 24例 SPF 级雄性 SD 大鼠随机分为三组，即预防组、模型组、正常组，每组8 例。统计分析三组大鼠的血清酶活性、血清透明质酸（HA）、层粘连蛋白（LN）、Ⅳ型胶原（IV-C）水平及血清、肝组织超氧化物歧化酶（SOD）、丙二醛（MDA）水平、肝纤维化程度、胶原总面积、面积比。结果：模型组、正常组、预防组大鼠的血清谷草转氨酶（AST）、佛甲草提取液（SLT）水平均逐渐降低（P <0.05），模型组、预防组、正常组大鼠的血清 HA、 LN、IV-C 水平均逐渐降低（P <0.05）。模型组、预防组大鼠的血清 SOD 水平均低于正常组（P <0.05）;模型组、正常组、预防组大鼠的血清、肝组织 MDA 水平逐渐降低（P <0.05），肝组织 SOD 水平逐渐升高（P <0.05）。模型组、预防组大鼠的肝纤维化0 级比例均低于正常组（P <0.05）;预防组大鼠的肝纤维化Ⅱ级比例高于模型组、正常组（P <0.05）;模型组大鼠的肝纤维化Ⅲ级比例高于正常组、预防组（P <0.05）。模型组、预防组、正常组大鼠的胶原总面积、面积比均逐渐降低（P <0.05）。结论：柴胡皂苷 d 抗肝纤维化机制可能和抗脂质过氧化作用相关。

【关键词】抗肝纤维化模型;大鼠;柴胡皂苷 d;脂质过氧化;肝纤维化程度;胶原总面积

【中图分类号】R258.5 R575【文献标识码】A 【文章编号】2096-5249（2021）18-0001-03

Analysis of effect of saikosaponin d on lipid peroxidation in rats with hepatic fibrosis

Jin Hong-yang1， Zhang Kun2， Hu Hai2， Sun Jie2

1.Baotou Medical College， Inner Mongolia University of science and technology， Baotou 014060， China;

2.Department of pathophysiology， Baotou Medical College， Inner Mongolia University of science and technology， Baotou 014060，China

【Abstract】Objective：To analyze the effect of saikosaponin d on lipid peroxidation in rats with hepatic fibrosis.Methods：24 SPF male SD rats were randomly divided into three groups：prevention group，model group and normal group，with 8 cases in each group.The levels of serum enzyme activity，serum hyaluronic acid（HA），laminin（LN），type Ⅳ collagen（Ⅳ-C），serum and liver superoxide dismutase（SOD），malondialdehyde（MDA），degree of liver ?brosis，total area and area ratio of collagen were statisti- callyanalyzed.Results：The levels of serum aspartate aminotransferase（AST） and SLT in model group，normal group and preven- tion group decreased gradually（P<0.05），and the levels of serum HA，LN and Ⅳ-C in model group，prevention group and normal group decreased gradually（P<0.05）.The levels of serum SOD in model group and prevention group were lower than those in normal group（P<0.05）;The levels of MDA in serum and liver tissue of model group，normal group and prevention group decreased grad- ually（P<0.05），and the level of SOD in liver tissue increased gradually（P<0.05）.The proportion of grade 0 liver fibrosis in model group and prevention group was lower than that in normal group（P<0.05）;The proportion ofgrade Ⅱ liver fibrosis in the prevention group was higher than that in the model group and normal group（P<0.05）;The proportion of grade Ⅲ liver fibrosis in model group was higher than that in normal group and prevention group（P<0.05）.The total area and area ratio of collagen in model group，pre- vention group and normal group decreased gradually（P<0.05）.Conclusion：The anti fibrosis mechanism of saikosaponin d may be related to its anti lipid peroxidation effect.

【Key words】Anti hepatic fibrosis model;Rats;Saikosaponin d;Lipid peroxidation;Degree of hepatic fibrosis;Total collagenarea

肝纤维化在分子水平上一方面和氧化应激相关分析关系密切，另一方面还和化学因子、细胞因子等关系密切[1]。相关促进其减慢。近年来，在兴起的自由基医学作用下，临床发现中药能够将鞣酸类、黄酮类等自由基成分清除，从而直接清除超氧阴离子，同时还能够促进机体抗氧化能力的增强，途径为对机体内氧化- 抗氧化系统进行调节[3]。……