口腔扁平苔藓患者血清和唾液中IL-1β、IL-5、IL-8、IL-12和 IFN-α的变化及临床意义

柳惠荣

[摘要] 目的 研究口腔扁平苔藓（OLP）患者血清和唾液中IL-1β、IL-5、IL-8、IL-12和 IFN-α浓度变化及其临床意义。 方法 選取2017年6月至2020年8月就诊于菏泽市中医医院口腔科门诊的31例患者，分为糜烂型（EOLP）和非糜烂型（NEOLP）两组，健康志愿者30名（对照组）。采用流式微球分析方法（CBA）应用流式细胞仪测定血清和唾液标本IL-1β、IL-5、IL-8、IL-12和 IFN-α浓度。 结果 分别与健康对照组比较，治疗前EOLP组、NEOLP组血清和唾液中IL-8细胞因子浓度差异有统计学意义（P<0.05），而EOLP组和NEOLP组比较，差异无统计学意义（P>0.05）;三组之间IL-1β、IL-5、IL-12和 IFN-α细胞因子浓度比较，差异无统计学意义（P>0.05）。三组血清和唾液各细胞因子浓度之间呈正相关，但相关程度较低（P>0.05）。 结论 血清和唾液中IL-8增高是促进OLP病理变化的部分原因。

[关键词] 扁平苔藓;口腔;白介素-1β;白介素-5;白介素-8;白介素-12;干扰素-α;流式微球分析方法

[中图分类号] R781.5          [文献标识码] A          [文章编号] 1673-9701（2021）30-0034-05

[Abstract] Objective To study the concentrations changes of IL-1β，IL-5，IL-8，IL-12 and IFN-α in serum and saliva of patients with oral lichen planus （OLP） and their clinical significance. Methods A total of 31 patients who were admitted to the stomatology clinic in Heze Hospital of Traditional Chinese Medicine from June 2017 to August 2020 were selected and divided into two groups：the erosive oral lichen planus （EOLP） group and the non-erosive oral lichen planus （NEOLP） group. Another 30 healthy volunteers were selected as the control group. The concentrations of IL-1β，IL-5，IL-8，IL-12 and IFN-α in serum and saliva specimens were determined by cytometric bead array （CBA） using a flow cytometer. Results According to the separate comparison of the healthy control group with EOLP group or the NEOLP group before treatment，there were statistically significant differences in terms of IL-8 cytokine concentrations in serum and saliva （P<0.05），while the difference between the EOLP group and the NEOLP group was not statistically significant （P>0.05）. There were no statistically significant differences in terms of concentrations of IL-1β，IL-5，IL-12 and IFN-α among the three groups （P>0.05）. There was low positive correlation in terms of correlation with cytokine concentrations in serum and saliva among the three groups （P>0.05）. Conclusion The increase in concentration of IL-8 in serum and saliva is part of the reason that promotes the pathological changes of OLP.

[Key words] Lichen planus; Oral cavity; Interleukin-1β; Interleukin-5; Interleukin-8; Interleukin-12; Interferon-α; Cytometric bead array

口腔扁平苔藓（Oral lichen planus，OLP）是一种常见的慢性口腔黏膜皮肤疾病，其发病与精神因素（如疲劳、焦虑、紧张）、免疫因素、内分泌因素、感染因素、微循环障碍等因素有关。一般根据病变黏膜的受损情况分为糜烂型（Erosive oral lichen planus，EOLP）和非糜烂型（Non-erosive oral lichen planus，NEOLP）。细胞因子主要包括一些小分子多肽，由多种免疫细胞分泌，在免疫应答过程中，细胞因子对于细胞之间相互作用、细胞的分化有重要调节作用。最近研究表明，OLP患者的黏膜损害因素中有细胞因子参与[1-2]。本研究应用流式细胞仪采用流式微球分析方法（Cytometric bead array，CBA）测定OLP患者血清和唾液中IL-1β、IL-5、IL-8、IL-12和 IFN-α细胞因子的水平，有利于更深入地了解OLP的发病机制，现报道如下。……