李翔宇 吴琳 窦勇 黄莹 翟胜利 周文礼




摘 要:为研究饲料中添加雨生红球藻对菲律宾蛤仔生长特性的影响,根据饵料中雨生红球藻与螺旋藻的比例设置5个梯度处理,分别为1∶9,3∶7,5∶5,7∶3,9∶1,投喂菲律宾蛤仔两周,监测各处理菲律宾蛤仔的肥满度及肝脏中多酚氧化酶(PPO)活性。结果表明,雨生红球藻与螺旋藻为5∶5的处理菲律宾蛤仔肥满度显著高于其他处理(P<0.05),而9∶1的处理显著低于其他处理(P< 0.05);雨生红球藻与螺旋藻为5∶5的处理菲律宾蛤仔PPO活性最高,其次是7:3的处理,二者显著高于1∶9的处理(P<0.05)。综合而言,菲律宾蛤仔的饵料中添加适量雨生红球藻粉有助于其生长及免疫性能提高,饵料中其与螺旋藻的比例5∶5为宜。
关键词:菲律宾蛤仔;雨生红球藻;肥满度;多酚氧化酶
中图分类号:S963.22 文献标识码:A DOI 编码:10.3969/j.issn.1006-6500.2021.12.005
Effects of Haematococcus Pluvialis on Plumpness and Polyphenol Oxidase of Philippine Clam
LI Xiangyu1, WU Lin1, DOU Yong1, HUANG Ying1, ZHAI Shengli1, ZHOU Wenli1
(1.Tianjin Key Laboratory for Aquaculture Ecdogy and Cultivation Fisheries College,Tianjin Agricultural University, Tianjin 300384, China;2.Tianjin Modern Chenhui Technology Group,Tianjin 301802,China)
Abstract: In order to study the effects of Haematococcus pluvialis on the growth characteristics of Philippine clam, the experiment set 5 gradient treatments basing on the proportion of Haematococcus pluvialis and Spirulina in the diet, which included 1∶9, 3∶7, 5∶5, 7∶3, and 9∶1. The Philippine clam was fed with the different treatment diets for two weeks, the plumpness and polyphenol oxidase (PPO) activity in liver of Philippine clam were monitored. The results showed that the clam's plumpness was significant higher than the other treatments when the ratio of Haematococcus pluvialis to Spirulina was 5∶5(P<0.05), and the Philippine clam's plumpness was significant lower than the other treatments when the ratio of Haematococcus pluvialis to Spirulina was 9∶1 (P<0.05). The PPO activity of Philippine clam were highest when the ratio of Haematococcus pluvialis to Spirulina was 5∶5, followed by the treatment of 7∶3, which the two treatments were significantly higher than the other treatment(P<0.05). In conclusion, adding appropriate amount of Haematococcus pluvialis in the diet of Philippine clam could approve the growth and specific immunity of Philippine clam, which the optimum ratio of Haematococcus pluvialis to Spirulina in the diet should be 5∶5.
Key words: Ruditapes philippinarum;Haematococcus pluviali;Plumpness;polyphenol oxidase
收稿日期:2021-08-04
基金項目:天津市企业科技特派员项目(20YDTPJC01470); 天津市水产生态及养殖重点实验室开放基金项目(TJAE201805);自然资源部海洋生态动力学重点实验室开放基金项目(MED202013);天津市宝坻区农业科技计划重大项目(201810);天津市科技局生态环境治理重大专项(18ZXSZSF00080); 天津市农业科技成果转化与推广项目(202001010)
作者简介:李翔宇(1996—),男,山西大同人,在读硕士生,主要从事生物饵料开发与应用方面研究。
通讯作者简介:窦勇(1985—),男,山东淄博人,讲师,博士,主要从事微藻生理生态方面研究。
菲律宾蛤仔(Ruditapes philippinarum)属于软体动物门双壳纲,营养价值高,肉质鲜美,含有许多人体必需的微量元素[1]。菲律宾蛤仔作为我国主要的海洋经济贝类之一,经济价值高,生长迅速,繁殖周期短,适应性较强,适合人工高密度养殖[2]。……