凤丹种子浸提液对白菜种子萌发及幼苗生长的影响

赵毅 石文昊 潘昊磊 张克亮

摘 要：为探讨凤丹种子是否存在抑制萌发的物质，以新成熟的凤丹种子作为试验材料，分别提取种皮和胚乳浸提液，研究不同浓度浸提液对白菜种子萌发、幼苗生长以及几种主要抗氧化酶活性的影响。结果表明，凤丹胚乳浸提液能显著抑制白菜种子的萌发与幼苗生长，抑制作用随着浸提液浓度的增加而增强，而种皮浸提液的抑制作用不显著，主要起到延缓幼苗生长的作用;凤丹胚乳浸提液显著抑制了白菜幼苗的POD和CAT酶活性，间接影响SOD酶活性。可见，凤丹种子中确实存在抑制种子萌发的内源抑制物，这些物质很可能是造成种子休眠的重要原因。

关键词：白菜;浸提液;抗氧化酶;抑制作用;种子休眠

中图分类号 S63 文献标识码 A 文章编号 1007-7731（2021）01-0025-04

Effects of Crude Extracts of Paeonia ostii Seeds on Germination and Activities of Antioxidant Enzyme of Brassica pekinensis

ZHAO Yi et al.

（College of Horticulture and Plant Protection，Jiangsu Key Laboratory of Crop Genetics and Physiology，Yangzhou University，Yangzhou 225009，China）

Abstract： To determine whether seeds of Paeonia ostii contain substance that inhibit seed germination， we studied the effect of different concentrations of P. ostii seed coat and endosperm crude extract on seed germination， seedling growth and several antioxidant enzyme activities of Brassica pekinensis. The results indicates that endosperm of P. ostii seeds could significantly inhibit seed germination and seedling growth of B. pekinensis， and the inhibitory effect increases with the increase of the extract concentration， while the seed coat extract has no significant inhibitory effect， and mainly delays the growth of seedlings. The endosperm extract of P. ostii seeds significantly inhibited the POD and CAT enzyme activities of B. pekinensis seedlings， and indirectly affect the SOD enzyme activity. Therefore， P. ostii seeds do have endogenous inhibitors that inhibit seed germination， and these substances are likely to be important reasons for seed dormancy.

Key words： Brassica pekinensis; Water crude; Antioxidant enzyme; Inhibiting effect; Seed dormancy

鳳丹（Paeonia ostii）是芍药科芍药属的一种多年生药用牡丹，主要分布在江南区域，具有重要的药用价值和经济价值[1]。凤丹根表皮中含丰富的丹皮酚，具有抗炎、解热和调节免疫系统的功效[2];大规模种植后发现，凤丹种子还具有重要的油用价值，凤丹籽油不饱和脂肪酸和α-亚麻酸含量高达92.26%、42%[3]。

凤丹主要以种子繁殖为主，但由于其种子具有较长的休眠期，育苗周期长，普遍存在发芽率低、出苗参差不齐的现象，严重影响了凤丹的生产和开发利用[4，5]。目前，对造成凤丹种子休眠原因的研究主要集中在发育不完全的种胚和生理后熟方面，而对种子内源性物质可能诱导种子休眠的研究则较少[5，6]。……