黔产五种卷柏黄酮含量测定及其对α-葡萄糖苷酶抑制活性研究

晏英 王丹 陈洁 唐攀 陈文章 廖伟科 杨小生

摘 要：建立中药卷柏特征成分总黄酮及穗花杉双黃酮的快速含量分析方法，比较不同卷柏药材中的含量及活性差异。采用紫外分光光度法和高效液相色谱法分别测定卷柏总黄酮和穗花杉双黄酮含量;通过建立体外α-葡萄糖苷酶抑制模型，对卷柏活性部位进行活性测试。不同卷柏中总黄酮含量在1.48%～2.67%之间，穗花杉双黄酮含量在0.49%～0.93%之间，活性部位的总黄酮含量在9.14%～16.46%之间，穗花杉双黄酮含量在2.26%～4.74%之间。卷柏活性部位的α-葡萄糖苷酶活性为：垫状卷柏IC50 6.56 μg/mL;疏叶卷柏IC50 4.47 μg/mL;兖州卷柏IC50 4.41 μg /mL;剑叶卷柏IC50 4.23 μg /mL;块茎卷柏IC50 3.12 μg/mL，均明显强于阳性对照阿卡波糖（IC50 76.82 μg/mL）。总黄酮和穗花杉双黄酮含量在不同品种间存在差异，其中对α-葡萄糖苷酶抑制作用和黄酮含量呈量效关系，且具有显著的α-葡萄糖苷酶抑制活性。

关键词：卷柏;总黄酮;穗花杉双黄酮;α-葡萄糖苷酶;抑制作用

Abstract：A rapid quantitative determination method of total flavonoids and amentoflavone，a characteristic component of the traditional Chinese medicine，was establisheto compare the content and activity differences in different Selaginella medicinal materials.The content of total flavonoidsand amentoflavone were determined by ultraviolet spectrophotometry and high performance liquid chromatography（HPLC），respectively.The activity test of the active part ofSelaginella was carried out through the establishment of α-glucosidase inhibition model in vitro. The results showed thatthe content of total flavonoids ranged from 1.48% to 2.67%，and the content of amentoflavone ranged from 0.49% to 0.93% in different plants. Meantime，the content of total flavonoidswas between 9.14% and 16.46%，and the content of amentoflavone ranged from 2.26% to 4.74% in the activity of five active parts. The α-glucosidaseactivity of five active partsofSelaginella，IC50 of S.pulvinate，S.remotifolia，S.involvens，S.xipholepis，S.chrysocauloswas6.56 μg/mL，4.47 μg/mL，4.41 μg/mL，4.23 μg/mL，3.12 μg/mL，respectively，which was significantly stronger than the positive control acarbose（IC50 76.82 μg/mL）.The contents of total flavonoids and amentoflavone weredifferentamongdifferent varieties.and the inhibitory effect on α-glucosidase was in a dose-effect relationship with the content of flavonoids，and has significant α-glucosidase inhibitory activity.

Keywords：Herba Selaginella;total flavonoids; amentoflavone; α-glucosidase; inhibitory activity

卷柏为蕨类植物门卷柏科（Selaginellaceae）卷柏属植物，约有700种。我国约有50种，分布全国各地，主产于湖南、福建、四川、陕西、江西、浙江等地[1]。卷柏为中国药典收载品种，卷柏属植物卷柏Selaginella tamariscina（Beauv.）Spring和垫状卷柏Selaginella pulvinate（Hook.et Grev.）Maxim.均作为卷柏入药[2]。现代药理及临床研究表明，卷柏具有抗炎、抗病毒、镇痛、降血压、降血糖、抗痛风和增强人体免疫功能等作用[3-8]。在化学成分研究中，卷柏中含有丰富的黄酮类成分，以双黄酮为主，其中双黄酮化学成分是以穗花杉双黄酮为代表的双黄酮类化合物[9-11]。研究表明，穗花杉双黄酮具有抗炎、抗氧化、抗肿瘤、抗病毒、抗真菌、抗老化、抗痴呆、降血糖、降血脂、抗抑郁、抗溃疡等生物活性[11-16]。……