饲料中莫昔克丁的含量测定

刘红云 吴昊 张莉 许世富 吴蕾 明文庆 蔡一杰 许晓靖

摘要 [目的]采用二极管阵列高效液相色谱法，建立饲料中莫昔克丁的检测方法。[方法]样品经乙腈溶液提取后，通过HLB 6cc SPE固相萃取小柱净化，C18色谱柱分离;以醋酸-醋酸铵缓冲液（pH 4.8）（取醋酸铵7.7 g，加水400 mL溶解后，用冰醋酸调节pH至4.8）-乙腈（400∶600）为流动相;检测波长为242 nm;柱温50 ℃;流速为2.5 mL/min。[结果]莫昔克丁在5～100 μg/mL具有良好的线性关系（R.2=0.999 0）。检出限为0.5 mg/kg，定量限为1.0 mg/kg。样品在定量限1倍、2倍、10倍3个加标水平下的平均回收率为95.0%～105.2%，批内、批间的相对标准偏差小于10%，日间和日内变异系数均低于15%。[结论]该试验建立的HPLC法简便、灵敏、准确，可用于饲料中莫昔克丁的含量测定。

关键词 饲料;莫昔克丁;含量测定;HPLC法

中图分类号 S816.17文献标识码 A文章编号 0517-6611（2021）07-0197-02

Abstract [Objective]A method for the determination of moxidectin feed by diode array high performance liquid chromatography （DADHPLC）was established.[Method]The sample was extracted by acetonitrile solution and purified by HLB 6cc SPE SPE solid phase extraction column. The mobile phase was acetic acidammonium acetate buffer （pH 4.8） （7.7 g ammonium acetate was dissolved in 400 mL water， and pH was adjusted to 4.8 with glacial acetic acid）acetonitrile （400∶600） as the mobile phase;the detection wavelength was 242 nm;the column temperature was 50 ℃;the flow rate was 2.5 mL/min. [Result]There was a good linear relationship in the range of 5-100 μg/mL （R.2=0.999 0）. The detection limit was 0.5 mg/kg and the limit of quantitation was 1.0 mg/kg. The average recoveries of the samples were 950% - 105.2% at 1， 2 and 10 times of the limit of quantification （LOQ）. The intra and inter assay relative deviations were less than 10%， and the interday and intraday coefficients of variation were less than 15%. [Conclusion]The HPLC method established in this experiment is simple， sensitive and accurate， and can be used to determine the content of moxidectin in feed.

Key words Feed;Moxidectin;Content determination;HPLC method

作者簡介 刘红云（1975—），女，安徽合肥人，高级兽医师，硕士，从事兽药、饲料、畜牧产品检测和研究工作。

莫昔克丁是一种新型抗寄生虫药，是由链霉菌发酵产生、成分单一、半合成的大环内酯类药物。莫昔克丁比阿维菌素类药物具有更好的驱虫活性，同时具有长效、安全等特性，且极低剂量下就有很强的抗线虫和节肢动物等体内外寄生虫活性，对猪体内的消化道线虫、肺线虫和体外寄生虫都有良好的驱杀效果[1-5]。20世纪80年代中期，莫昔克丁开始作为兽用驱虫药使用。作为新一代驱虫药物，莫昔克丁能够高效地杀灭线虫和体表寄生虫，同时对动物有很好的安全性。现有的杀虫剂伊维菌素、阿维菌素等异味较大，施用后无论是对于动物还是使用者而言都有较为严重的感官刺激。……