饵料强化剂对牙鲆苗种繁育期存活及生长的影响

赵春暖 任利群 于涛 蔡忠强 郑言鑫 王喜文 陈洋

摘要 以裂壶藻为饵料强化剂，设置不同的裂壶藻强化剂使用量及强化时间，研究其对繁育期牙鲆苗种生长发育的影响。结果表明，轮虫强化密度为450个/mL，卤幼强化密度为150个/mL。当轮虫强化剂使用量为0.09 g/L，强化时间12 h，卤幼饵料强化剂使用量为0.10 g/L、强化时间20 h时，牙鲆苗种的发育、驯化情况最好，存活率最高，生长速度最快。通过对强化持续期的对比发现，强化饵料投喂20 d时牙鲆苗种变态存活率趋于稳定，无白化、病害发生，个体生长均匀、生长速度快。

关键词 牙鲆;轮虫;卤虫;强化剂使用量

中图分类号 S961文献标识码 A

文章编号 0517-6611（2021）10-0089-03

doi：10.3969/j.issn.0517-6611.2021.10.024

开放科学（资源服务）标识码（OSID）：

Effects of Diet Enhancer on the Survival and Growth of Paralichthys olivaceus Seedlings during the Breeding Period

ZHAO Chun-nuan，REN Li-qun，YU Tao et al （Changdao Multiplication Experimental Station，Chinese Academy of Fishery Sciences，Yantai，Shandong 265800）

Abstract Taking Schizochytrium sp. as bait enhancer，different enhancer dose and intensification time were set up to study their effects on the growth of Paralichthys olivaceus seedlings during the breeding period.The enhanced density of rotifer was 450 ind./mL，and the density of artemia was 150 ind./mL.When the enhanced dose of rotifer was 0.09 g/L，the intensification time was 12 h，the enhanced dose of artemia was 0.10 g/L，the intensification time was 20 h，the development and domestication of P.olivaceus seedlings were the best，the survival rate was the highest，and the growth rate was the fastest.Through the comparison of the strengthening duration，it was found that the metamorphic survival rate of P.olivaceus seedlings tended to be stable after P.olivaceus seedlings were fed with the bait enhancer 20 days，no albinism or disease occurred，the individual grew evenly and the growth rate was fast.

Key words Paralichthys olivaceus;Rotifers;Artemia;Enhanced dose

在牙鲆苗種繁育过程中，饵料强化剂发挥着重要作用，影响牙鲆苗种的正常发育，是饵料驯化过程中必不可少的重要材料。笔者立足牙鲆海水鱼类的饵料营养强化，开展牙鲆繁育各个阶段的开口饵料营养强化剂使用量、营养强化持续期研究，以期为海水鱼类繁育工作提供有效参考，丰富牙鲆健康繁育技术，提高牙鲆存活率、生长速度，有效增强增殖放流海水鱼苗种的放流品质和放流成效。研究表明，投喂未经营养强化的轮虫、卤虫无节幼体不利于苗种的成活、生长以及抗病、抗逆能力[1-3]。雷霁霖院士是工厂化育苗与养殖产业化的主要奠基人和学科带头人。研究发现，海水仔稚鱼对脂类营养具有一定的需求[4]。裂壶藻粉是由裂殖壶菌的紫外诱变菌株经发酵培养、干燥发酵液得到的藻粉。……