秋茄叶片愈伤组织褐变研究

池上评

摘要 [目的]建立红树秋茄的愈伤组织体系，为实现秋茄种质资源离体保存及其多样性研究提供科学依据。[方法]以红树林秋茄幼嫩叶片作为外植体，研究2，4-D和6-BA不同浓度配比下秋茄叶片愈伤组织的诱导率，以及不同激素水平对秋茄叶片和愈伤组织褐变情况的影响。[结果]2，4-D对秋茄叶片愈伤组织形成是必需的，添加一定量的6-BA有助于提高愈伤组织诱导率，最佳激素配比为MS+1.0 mg/L 2，4-D+1.0 mg/L 6-BA，诱导率最高，达35.0%;秋茄叶片和愈伤组织均有褐变现象发生，21 d内，褐变率达33.3%～45.0%。[结论]初步明确了秋茄叶片愈伤组织的诱导条件及褐变影响因素。

关键词 秋茄;愈伤组织;继代培养;褐变

中图分类号 S792.99 文献标识码 A 文章编号 0517-6611（2021）09-0130-03

doi：10.3969/j.issn.0517-6611.2021.09.033

Abstract [Objective] To establish the callus system of Kandelia candel and provide scientific basis for in vitro conservation and diversity research of Kandelia candel germplasm resources. [Method] Taking young leaves of Kandelia candel as explants， the callus induction rate of Kandelia candel leaves under different concentrations of 2，4-D and 6-BA， and the effects of different hormone levels on the browning situation of Kandelia candel leaves and callus were studied. [Result] 2，4-D was necessary for callus formation of Kandelia candel leaves. Adding a certain amount of 6-BA was helpful to improve the callus induction rate. The best hormone ratio was MS+1.0 mg/L 2，4-D+1.0 mg/L 6-BA， and the induction rate was up to 35.0%. The leaves and callus of Kandelia candel had browning phenomenon， and the browning rate reached 33.3% - 45.0% within 21 days. [Conclusion] The callus induction conditions and browning influencing factors of Kandelia candel leaves were preliminarily determined.

Key words Kandelia candel;Callus;Subculture;Browning

红树林是我国东南沿海的木本植物群落，对调节全球的气候变化具有重要意义[1-2]，又因其有着丰富的生物量而具有巨大的生态价值[3-4]。同时，完整的红树林不仅可以为人类提供良好的游憩场所，还是一个药源宝库，因此具有很高的社会价值和经济价值[5-6]。秋茄（Kandelia candel）是红树林的主要造林树种[6]，具有重要的生态保护价值。目前，秋茄的自然分布北界位于福建北部（27°20′N），浙江温州有少量人工栽培[7-9]。秋茄一般采用胚轴（胎生苗）直接插植进行繁殖，不仅繁殖方式单一，还存在繁殖周期较长、效率低、可控性差、人工移植成活率低、成本高及不易大面积推广等问题。随着组织培养技术在林业上的应用[10]，研究如何利用这一技术来解决秋茄扩大繁殖具有重要意义。目前，关于秋……