清热祛瘀汤对慢性盆腔炎性疾病模型大鼠的影响

杨成成 季晓黎 文怡 尹小兰 杨伊斯

摘要 目的：探討清热祛瘀汤对慢性盆腔炎性疾病模型大鼠的炎性反应细胞因子及粘连相关免疫分子的影响。方法：选取100只Wistar雌性大鼠为研究对象，以随机分组方式将其分为5组，分别为模型对照组、假手术组、清热祛瘀汤组（包括低、中、高3种不同剂量），每组20只;除假手术组外，其余5组均建立盆腔炎性疾病大鼠模型;于清热祛瘀方作用下对其炎性反应细胞因子、血液流变学、粘连相关免疫分子变化情况进行分析。结果：与假手术组比较，模型对照组大鼠血液流变学各指标均明显升高变（P<0.05）;而经治疗后，各指标均明显下降，且与模型对照组比较差异均有统计学意义（P<0.05）;模型对照组与假手术组比较，大鼠血清IL-4、IL-10、TNF-α、IgA、IgG差异均有统计学意义（P<0.05）;经治疗后，清热祛瘀方各剂量组各指标水平均发生明显改变（P<0.05）。结论：清热祛瘀汤对慢性盆腔炎性疾病模型大鼠的炎性反应细胞因子及粘连相关免疫分子、血液流变学均有较大影响，可推广应用。

关键词 清热祛瘀汤;慢性盆腔炎性疾病;炎性反应细胞因子;血液流变学;免疫分子;大鼠;粘连相关免疫分子;假手术

Experimental Study on Qingre Quyu Decoction on Inflammatory Cytokines and Adhesion-associated

Immune Molecules in Rats with Chronic Pelvic Inflammatory Disease

YANG Chengcheng，JI Xiaoli，WEN Yi，YIN Xiaolan，YANG Yisi

（Department of Gynecology，Affiliated Hospital of Chengdu University of Traditional Chinese Medicine，Chengdu 610072，China）

Abstract Objective：To investigate the effects of Qingre Quyu Decoction on inflammatory cytokines and adhesion-related immune molecules in rats with chronic pelvic inflammatory disease.Methods：One hundred female Wistar rats were selected as subjects.They were randomly divided into five groups：model control group，sham operation group and Qingre Quyu Decoction group（including three different doses of low，medium and high），with 20 rats in each group.Except for the sham operation group，the other 5 groups established a rat model of pelvic inflammatory disease; the changes of inflammatory cytokines，hemorheology and adhesion related immune molecules were analyzed under the action of Qingre Quyu Decoction.Results：Compared with sham operation group，the indexes of hemorheology of the model control group were significantly increased（P<0.05）.After treatment，the indexes were significantly decreased，and the differences were Statistically significant compared with the model control group（P<0.05）.There were statistically significant differences in serum IL-4，IL-10，TNF-α，IgA and IgG between model control group and sham operation group（P<0.05）; After treatment，the levels of of indexes in each dose group of Qingre Quyu Decoction significantly changed（P<0.05）.Conclusion：Qingre Quyu Decoction has great influence on inflammatory cytokines，adhesion related immune molecules and hemorheology in chronic pelvic inflammatory disease rats，and can be popularized and applied.

Keywords Qingre Quyu Decoction; Chronic pelvic inflammatory disease; Inflammatory cytokines; Hemorrheology; Immune molecules; Rats; Adhesion-related immune molecules; Sham operation

中图分类号：R285.5文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.02.013

盆腔炎性疾病（Pelvic Inflammatory Diseases，PID）为女性常见疾病，为性活跃期女性发生率较高的一种上生殖道感染性疾病[1-2]。急性期若未得到有效治疗，则极易因病程迁延、患者体质差等因素而逐渐形成慢性盆腔炎性疾病（CPID）。而慢性盆腔炎性疾病的病情较为顽固，极易导致患者出现月经不调、白带异常增多、腰腹疼痛等症状;且慢性盆腔炎性疾病还可引发盆腔静脉曲张，从而诱发盆腔淤血综合征，严重时甚至可引发不孕，严重影响女性健康[3-4]。如何有效改善患者病情，提高女性生命质量已成为临床关注的重点。既往，临床上通常将现代医学抗感染等药物作为治疗该病的常用手段，但效果并不理想。且随着广谱抗生素的应用，致使药物耐药性不断升高[5-6]。……