清开灵注射液对急性脑缺血大鼠不规则趋化因子表达的影响

刘豫玥 闫杨杨 狄波 潘彦舒

摘要 目的：观察清开灵注射液对大鼠脑梗死缺血性损伤急性期阶段，不规则趋化因子（Fractalkine，FKN）的表达变化，探讨清开灵注射液治疗急性脑缺血的可能机制。方法：线栓法制备大鼠大脑中动脉永久性梗死模型（MCAO），随机分为假手术组、模型组，清开灵注射液观察组，TTC染色观察大鼠治疗前后脑组织梗死体积变化，同时采用蛋白芯片、ELISA和免疫组化法分别检测脑脊液、外周血清及脑组织中FKN的表达水平。结果：脑组织TTC染色顯示模型组均出现明显梗死灶，清开灵观察组的梗死体积明显缩减。与模型组比较，清开灵观察组明显下调病变局部脑组织的FKN（P<0.05），且能明显上调血清中的FKN（P<0.05），同时观察到其可上调脑脊液及全脑的FKN，差异无统计学意义（P>0.05）。结论：清开灵注射液能缩小脑梗死体积，减轻急性脑缺血性损伤，其机制可能与调节FKN的表达有关。

关键词 急性脑缺血;不规则趋化因子;清开灵注射液;大鼠

Effect of Qingkailing Injection on the Expression of Irregular Chemokines in Rats with Acute Cerebral Ischemia

LIU Yuyue1，YAN Yangyang2，DI Bo3，PAN Yanshu4

（1 Dongfang Hospital Affiliated to Beijing University of Chinese Medicine，Beijing 100078，China; 2 Dongzhimen Hospital

Affiliated to Beijing University of Chinese Medicine，Beijing 100070，China; 3 China Academy of Chinese medical

Science，Beijing 100070，China; 4 Beijing University of Chinese Medicine，Beijing 100029，China）

Abstract Objective：To observe the expression changes of irregular chemokine （Fractalkine，FKN） in the acute phase of ischemic injury of rat cerebral infarction by Qingkailing Injection，and to explore the possible mechanism of Qingkailing Injection in the treatment of acute cerebral ischemia.Methods：A rat model of permanent middle cerebral artery infarction （MCAO） was prepared by suture method and randomly divided into a sham operation group，a model group，and a Qingkailing Injection treatment group.TTC staining was used to observe the changes in infarct volume of rat brain tissue before and after treatment.Protein chip，ELISA and immunohistochemistry were used to detect the expression level of FKN in cerebrospinal fluid，peripheral serum and brain tissue.Results：TTC staining of brain tissue showed that there were obvious infarcts in the model group，and the infarct volume in the Qingkailing treatment group was significantly reduced.Compared with the model group，the Qingkailing treatment group significantly down-regulated the FKN of the diseased local brain tissue （P<0.05），and significantly increased the FKN in the serum （P<0.05）.It was observed that it could up-regulate the cerebrospinal fluid and the whole brain FKN.But the difference was not statistically significant （P>0.05）.Conclusion：Qingkailing Injection can reduce the volume of cerebral infarction and reduce acute cerebral ischemic injury.The mechanism may be related to the regulation of FKN expression.

Keywords Acute cerebral ischemia; Fractalkine; Qingkailing Injection; Rat

中图分类号：R285.5;R743文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.02.014

急性脑缺血损伤可继发引起炎性反应及应答，产生的一系列炎性因子，其始动环节离不开一系列趋化因子的参与。不规则趋化因子（Fractalkine，FKN）是家族中较少的在中枢神经系统中组成性表达的趋化因子之一，近年来在急性脑缺血性损伤中的作用也逐渐受到关注。FKN主要集中分布在大脑皮质、海马、尾状壳核、丘脑、嗅球[1]，缺血再灌注损伤的脑海马中FKN信号显著增加，抑制CX3CR1受体功能，进一步激活更多的小胶质细胞及炎性反应因子表达水平，加重中枢神经炎性反应及认知损害[2]，而炎性反应的程度与急性脑缺血损伤程度密切相关。……