柏艾胶囊对不同类型高血压模型降压作用的研究

赵荣华 王道涵 曲天歌 孙静 包蕾 高英杰 崔晓兰

摘要 目的：探討柏艾胶囊对自发性高血压模型、卒中型自发性高血压模型及肾动脉狭窄性高血压模型大鼠血压的影响。方法：将大鼠随机分为正常血压大鼠（wky）对照组/假手术组、模型对照组、卡托普利阳性药对照组（5.4 mg/kg）、柏艾胶囊大、中、小剂量组（6.42 g/kg、3.24 g/kg、1.62 g/kg），采用自动血压记录仪测量给药前后大鼠尾动脉血压。结果：柏艾胶囊大、中、小剂量组（6.42 g/kg、3.24 g/kg、1.62 g/kg）均可不同程度降低SHR模型大鼠的血压值，与模型对照组比较，给药2周后各给药组血压值降低，且差异有统计学意义（P<0.01），停药4周后血压值仍见不同程度降低，且差异有统计学意义（P<0.01）;柏艾胶囊大、中剂量给药组（6.42 g/kg、3.24 g/kg）可不同程度降低SHRsp模型大鼠血压值，与模型对照组比较，给药1周后血压值明显降低，且差异有统计学意义（P<0.01），停药4周后柏艾胶囊大剂量组（6.42 g/kg）血压值仍显著降低，且与模型对照组比较，差异有统计学意义（P<0.01）;柏艾胶囊大、中、小剂量组（6.42 g/kg、3.24 g/kg、1.62 g/kg）均可不同程度降低肾动脉狭窄模型高血压大鼠血压值，与模型对照组比较，给药1周后，各给药组血压值降低，且差异有统计学意义（P<0.01）。结论：柏艾胶囊具有降低原发性高血压和肾性高血压的作用。

关键词 柏艾胶囊;自发性高血压模型;卒中型自发性高血压模型;肾动脉狭窄性高血压模型;靶器官;不良反应;肝阳上亢型;肝肾阴虚

Study on the Antihypertensive Effect of Boai Capsule on Different Types of Hypertension Model

ZHAO Ronghua1，WANG Daohan2，QU Tiange3，SUN Jing1，BAO Lei1，GAO Yingjie1，CUI Xiaolan1

（1 Institute of Chinese Meteria Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China; 2 Dongfang Hospital，Beijing University of Chinese Medicine，Beijing 100078; 3 Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100700，China）

Abstract Objective：To evaluate Boai Capsule on rat blood pressure effects of spontaneously hypertensive model，type of spontaneously hypertensive stroke model，and renal artery stenosis hypertensive model.Methods：Rats were randomly assigned into a wky group or sham-operation group，a model control group，a captopril positive medicine control group （5.4 mg/kg），a large dose，a medium dose and a low dose of Boai Capsule group （6.42 g/kg，3.24 g/kg，1.62 g/kg）.Before and after dosing Bo-Ai Capsule，the rat tail artery blood pressure was measured by using automatic blood pressure recorder.Results：The large，medium，and low dose groups （6.42 g/kg，3.24 g/kg，1.62 g/kg） of Boai Capsule can reduce the blood pressure value of SHR model rats to varying degrees.Compared with the model control group，after 2 weeks of administration，the blood pressure value of each administration group decreased，and the difference was statistically significant （P<0.01）.The blood pressure value still decreased to varying degrees after stopping the drug for 4 weeks，and the difference was statistically significant （P<0.01）.The large and medium dose administration group of Boai Capsule （6.42 g/kg，3.24 g/kg） can reduce the blood pressure value of SHRsp model rats to different degrees.Compared with the model control group，the blood pressure value was significantly reduced after 1 week of administration，and the difference was statistically significant （P<0.01）.The blood pressure value of the large dose of Boai Capsule group （6.42 g/kg） was still significantly reduced after 4 weeks of discontinuation，and the difference was statistically significant compared with the model control group （P<0.01）.Compared with model group，the blood pressure of renal artery stenosis hypertensive model rats decreased significantly in large，medium and low dose groups （6.42 g/kg、3.24 g/kg，1.62 g/kg） of Boai Capsule after dosing one week and the difference was statistically significant （P<0.01）.Conclusion：Boai Capsule had effects of decreasing blood pressure of spontaneously hypertensive and renal artery stenosis hypertensive models.

Keywords Boai Capsule; Spontaneous hypertension model; Stroke medium spontaneous hypertension model; Renal artery stenosis hypertension model; Target organ; Toxic and side effects; Hyperactivity of liver yang; Deficiency of liver and kidney yin

中圖分类号：R285.5文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.06.010

柏艾胶囊源于传统古方，具有滋阴凉血、泻火平肝之功效，在多年的临床应用中显示，对阴虚阳亢、肝火亢盛型高血压有显著疗效，前期实验也研究了其对犬血流动力学的影响[1]，为进一步证实其治疗高血压的疗效，我们又进行了进一步的动物实验，以便为临床应用提供实证依据。

1 材料与方法

1.1 材料

1.1.1 动物 自发性高血压模型大鼠（SHR），3个月龄，体质量（210±20）g，雌雄各半，购自上海第二医科大学实验动物科学部，合格证号：医动字第02-23-6号;卒中型自发性高血压模型大鼠（SHRsp），3个月龄，体质量（210±20）g，雌雄各半，购自中国医学科学院北京阜外医院;维斯塔尔（Wister）大鼠，体质量（230±10）g，购自中国医学科学院实验动物研究中心，合格证号SCXK11-00-0006。实验条件：屏障环境，SPF级，恒温，温度24 ℃，恒湿，湿度55%，饲养，自由进水和食物。……