万寿菊中叶黄素的超高压提取工艺优化

刘冰雪 王玲玲 张晓雪 耿宇婷 康荷笛 赵修华

摘 要：为研究万寿菊中叶黄素的提取工艺，本研究以东北万寿菊花为提取原料，采用超高压法提取万寿菊花中叶黄素并对其提取工艺进行优化。采用单因素和响应面实验，考察了4种不同因素料液比、提取次数、保压时间及提取压力对叶黄素提取率的影响，最终得到最佳提取工艺条件为：料液比为1∶95.21（g/mL），提取次数为4次，保压时间为3 min，提取压力为200 MPa，在此条件下进行3次重复实验，得到叶黄素实际提取率可达（68.57±2.31） mg/g，与预测值69.38 mg/g一致。与热回流提取法和微波-超声协同提取法相比，超高压技术提取万寿菊花中叶黄素的提取率可以显著提高。研究结果表明，超高压技术提取万寿菊花中的叶黄素提取稳定性好、工艺合理可靠，并且重复性强，可广泛应用。

关键词：万寿菊;叶黄素;超高压提取;工艺优化;响应面

中图分类号：R914    文献标识码：A   文章编号：1006-8023（2021）04-0071-08

Optimization of Ultra-High Pressure Extraction Process

for Lutein from Marigold

LIU Bingxue， WANG Lingling， ZHANG Xiaoxue， GENG Yuting， KANG Hedi， ZHAO Xiuhua*

（College of Chemistry， Chemical Engineering and Resource Utilization， Northeast Forestry University， Harbin 150040， China）

Abstract：In order to study the extraction technology of lutein from marigold， this study used northeast marigold as raw material to extract lutein from marigold by ultra-high pressure method and optimized the extraction technology. The single factor experiment and response surface were used to investigate the effects of four different extraction methods， such as solid-liquid ratio， extraction times， holding time and extraction pressure， on the extraction yield of lutein from marigold. Finally， the optimal extraction conditions were obtained as follows： the solid-liquid ratio was 1∶95.21（g/mL）， the extraction times was 4 times， the pressure holding time was 3 min， and the extraction pressure was 200 MPa. Under these conditions， the experiment was repeated for three times. The actual extraction yield of lutein was（ 68.57±2.31）mg/g， which was consistent with the predicted value of 69.38 mg/g. Compared with thermal reflux extraction and microwave-ultrasonic extraction， the extraction rate of lutein from marigold could be significantly improved by ultrahigh pressure extraction. The results showed that the ultra-high pressure extraction of lutein from marigold was stable， reliable and reproducible， and could be widely used.

Keywords：Marigold; lutein; ultra-high pressure extraction; process optimization; response surface

收稿日期：2021-03-12

基金項目：黑龙江省杰出青年基金项目 （JC2018005）

第一作者简介：刘冰雪，硕士研究生。研究方向为药物化学。E-mail： 792224304@qq.com

*通信作者：赵修华，博士，教授。研究方向为植物化学与植物药。E-mail： xiuhuazhao@nefu.edu.cn

引文格式：刘冰雪， 王玲玲， 张晓雪， 等. 万寿菊中叶黄素的超高压提取工艺优化[J].森林工程， 2021，37（4）：71-78.

LIU B X， WANG L L， ZHANG X X， et al. Optimization of ultra-high pressure extraction process for lutein from marigold [J]. Forest Engineering，2021，37（4）：71-78.

0 引言

叶黄素是自然界中的一种天然色素，属类胡萝卜素家族，分子内含有多个不饱和双键，从而使其具有较强抗氧化性[1]。叶黄素具有诸多的药理活性，包括预防和治疗多种疾病（黄斑病变、青光眼等）有重要作用[2]，同时也可以增强老年人的认知能力。……