茶树WOX基因家族的鉴定和表达模式分析

艾安涛 张宝会 彭徐乾 吕立堂

摘要：WOX转录因子在植物的生长发育和非生物胁迫响应中起着重要的调控作用。文章基于全基因组数据，从茶树基因组中鉴定出29个WOX基因，并对其基因结构、进化关系、保守域、染色体定位进行分析，同时分析了它们在PEG诱导的干旱胁迫、盐胁迫处理中的转录组数据。结果表明，29个CsWOX（茶树WOX）基因在茶树染色体上分布不均;根据进化关系将茶树WOX基因分为4类;基因结构和保守基序分析发现相同亚家族的基因结构和保守结构域基本一致;基因表达分析显示，CsWOX基因在花和果实组织中具有较高的表达水平，且部分基因随着叶片成熟度的增加，表达水平升高;不同的CsWOX基因在PEG诱导的干旱胁迫、盐胁迫处理下存在差异表达，说明CsWOX基因广泛参与茶树生长发育并在响应非生物胁迫中发挥重要作用。该结果将为进一步研究WOX基因在调控茶树生长发育和非生物胁迫响应中的作用提供一些有价值的信息，为茶树WOX基因的功能研究与利用提供科学依据。

关键词：茶树;WOX基因家族;转录因子;基因表达分析

Identification and Expression Pattern Analysis of

WOX Gene Family in Camellia sinensis （L.）

AI Antao1， ZHANG Baohui2， PENG Xuqian1， L    Litang1，2*

1. College of Tea Science， Guizhou University， Guiyang 550025， China; 2. Institute of Agro-Bioengineering， Guizhou

University College of Life Sciences， Key Laboratory of Plant Resources Conservation and Germplasm

Innovation in Mountainous Regions， Ministry of Education， Guiyang 550025， China

ü

Abstract： WOX transcription factors play an important regulatory role in plant growth， development and response toabiotic stresses. Based on the tea whole genome data， this study identified 29 WOX genes and analyzed their genestructures， evolutionary relationship， conserved domains， and chromosome locations. Meanwhile， their responses to drought stress and salt stress induced by PEG were studied. The results show that 29 CsWOX genes were unevenlydistributed on tea plant chromosomes. They were classified into category IV according to evolutionary relationship. Their gene structures and conserved domains of the same subfamily were basically the same. Gene expression analysisshows that the CsWOX genes had higher expression levels in flower and fruit tissues， and the expressions of somegenes increased with the increase of leaf maturity. CsWOX genes exhibited different expression patterns underPEG-induced drought stress and salt stress， indicating that CsWOX genes are widely involved in tea plant growth anddevelopment and play different roles in response to abiotic stress. This result will provide some valuable informationfor further research on the role of WOX gene in regulating the growth and development of tea plants and response toabiotic stresses. It also provided a scientific basis for the functional research and utilization of tea plant WOX genes.

Keywords： Camellia sinensis （L.）， WOX gene family， genome identification， gene expression analysis

WOX蛋白是植物特有的一類转录因子，其名称源于一段由约60个氨基酸组成的高度保守的螺旋-环-螺旋-拐角-螺旋结构域，此外，在WUS组或者I组的WOX蛋白氨基酸C端还存在一个高度保守的基序，命名为WUS-box （TLXLTP），该基序在WOX蛋白调控植物生长发育进程中起重要的作用[1]。

近几年，研究者通过正向遗传和反向遗传方法对不同植物的研究表明，WOX转录因子……