姬松茸-灰树花-蛹虫草复合多糖对免疫低下小鼠免疫调节的影响

马传贵 张志秀 李红芳

【摘要】目的：以姬松茸-灰树花-蛹虫草制成的复合多糖，以免疫抑制小鼠细胞免疫功能为主要指标，探究比较复合多糖与各单一多糖对免疫低下小鼠免疫调节功能影响的差别。方法：通过检测小鼠细胞免疫功能和体液免疫功能，并对相关指标进行检验。结果：复合多糖组小鼠的体质量高于同性别的其他单一多糖组；各多糖组小鼠胸腺系数均有不同程度的升高，其中复方多糖组效果最好；复合多糖组IgG、IgA、IgM、sIgA水平升高效果，較单一多糖组血清好；在血液、脾脏T细胞各亚群及比值方面，复合多糖升高较好，血液CD4-CD8+降低效果亦优于单一多糖组；复合多糖组T淋巴细胞增殖率高于单一多糖组，差异有统计学意义（P<0.05）。结论：复合多糖对小鼠细胞免疫、体液免疫和T淋巴细胞功能有较好的改善作用，能显著改善免疫抑制小鼠的免疫功能。

【关键词】姬松茸；灰树花；蛹虫草；复合多糖；小鼠；免疫功能

[中图分类号]R285.5 [文献标识码]A [文章编号]2096-5249（2021）03-0005-04

Effects of compound polysaccharides from Agaricus blazei murrill- Griflola frondosa-Cordyceps militaris on the immunological function in immunosuppressive mice

MA Chuan-gui1， ZHANG Zhi-xiu1， LI Hong-fang2* （1. Beijing Jingcheng Biotechnology Company Limited， Beijing 102600， Chnia； 2. Yunnan University of Chinese Medicine，Kunming Yunnan 650500， Chnia）

[Abstract] Objective： To compare the effect of compound polysaccharide and single polysaccharide on the immunoregulation of immunocompromised mice. Methods： the cellular immune function and humoral immune function of mice were tested. Results： the body weight of mice in compound polysaccharide group was higher than that of other single polysaccharide groups of the same sex； the thymus coefficient of mice in each polysaccharide group increased to different degrees， and the effect of compound polysaccharide group was the best； the effect of compound polysaccharide group was better than that of single polysaccharide group in serum IgG， IgA， IgM， SIgA； the effect of compound polysaccharide was better than that of single polysaccharide in blood CD4 / CD8， spleen CD4 / CD8， and blood CD4-CD8 +The results showed that the effect of decreasing was good， the effect of increasing T-lymphocyte proliferation rate of compound polysaccharide group was better than that of single polysaccharide group， and the difference was statistically significant （P<0.05）. Conclusion： the compound polysaccharide has a better effect on improving the cellular immunity， humoral immunity and T lymphocyte function of mice， and can significantly improve the immune function of immunosuppressed mice.

[Key words] Agaricus blazei murrill； Griflola frondosa； Cordyceps militaris； Compound polysaccharides； mice； immunological function

多糖广泛存在于动植物以及真菌体内，组成和结构上的差异亦使其生物学活性复杂多样，植物多糖分子存在2-10个单糖聚合而成的寡糖片段，通过与蛋白受体结合，激活巨噬细胞、自然杀伤细胞和树突状细胞（dendritic cell，DC）等免疫细胞表面存在的多糖受体，产生类似酶作用过程的效果，进而影响淋巴细胞基因表达，促使T、B淋巴细胞的增殖，调节细胞因子分泌、活化补体，促进抗体产生等[1][2][3]。姬松茸多糖有广泛的药理活性，在抗肿瘤、增强免疫力、抗疱疹病毒、影响造血功能等方面优势突出[4]；……