小金蝠蛾幼虫β-1，3-葡聚糖识别蛋白βGRP1基因的分子特征与生物信息学分析

贺元川 罗昌树 游华建 陈仕江

摘要 [目的]探索冬蟲夏草寄主昆虫小金蝠蛾幼虫的β-1，3-葡聚糖识别蛋白βGRP1基因的结构特征与进化关系。[方法]通过PCR法克隆获得小金蝠蛾幼虫βGRP1基因的编码序列，并采用Protparam、NetPhos 2.0 Server cDNA、MEGA等软件分析βGRP1蛋白的理化特征和进化分析。[结果]小金蝠蛾幼虫βGRP1基因编码序列1 593 bp，推断可编码530氨基酸组成的蛋白，该蛋白为分泌型蛋白，含有20个氨基酸的信号肽，包含2个功能结构域，该结构域与葡聚糖的识别密切相关;同源分析和分子系统进化树分析表明，鳞翅目昆虫中的βGRPs基因家族分为4个进化分枝，其中小金蝙蝠蛾与勾蝠蛾为单独的一个进化分枝，小金蝠蛾幼虫βGRP1进化较钩蝠蛾早，介于I型和Ⅲ型分支。[结论]该蛋白具有识别β-1，3-葡聚糖的结构域，并可能通过丝氨酸磷酸化激活酚酶源级联系统，启动免疫，为进一步探索βGRP1蛋白的免疫功能奠定基础。

关键词 冬虫夏草;小金蝠蛾;β-1，3-葡聚糖识别蛋白;先天免疫

中图分类号 Q 966  文献标识码 A  文章编号 0517-6611（2021）19-0095-04

doi：10.3969/j.issn.0517-6611.2021.19.023

开放科学（资源服务）标识码（OSID）：

Molecular Characteristics and Bioinformatics Analysis of β-1，3-glucan Recognition Protein βGRP1 Gene of  Hepialus xiaojinensis  Larvae

HE Yuan-chuan1，2， LUO Chang-shu1， YOU Hua-jian1，2 et al

（1. Chongqing Academy of Chinese Materia Medica， Chongqing 400065; 2. Gongga Chinese Caterpillar Fungus Industry Co.， Ltd.， Kangding， Sichuan 626000）

Abstract [Objective]The study aimed to explore the structural characteristics and evolutionary relationship of the β-1， 3-glucan recognition protein βGRP1 gene of the larvae of the host insect  Hepialus xiaojinensis . [Method]The coding sequence of the βGRP1 gene of  Hepialus  larvae was cloned by PCR， and the physical and chemical characteristics and evolutionary analysis of βGRP1 protein were analyzed by Protparam， NetPhos 2.0 Server cDNA， MEHA and other methods. [Result]The coding sequence of βGRP1 of the larvae of  Hepialus xiaojinensis  is 1 593 bp， which is inferred to encode a protein composed of 530 amino acids. This protein is a secreted protein， contains a signal peptide of 20 amino acids and contains 2 functional domains， which probably combined with dextran. The analysis results of molecular phylogenetic tree showed that the  Lepidopteran  insects βGRPs gene family was divided into 4 evolutionary branches. The evolution of βGRP1 of the larvae of  Hepialus xiaojinensis  is earlier than that of Hepialus pu， which lies between type I and Ⅲ. [Conclusion] The study indicates that the protein has a domain that recognizes β-1， 3-glucan， and may activate the phenolase-derived cascade system through serine phosphorylation to initiate immunity， which supply protein foundation for research the immune function of βGRP1.

Key words  Ophiocordyceps sinensis ; Hepialus xiaojinensis ;β-1， 3-glucan recognition protein;Innate immunity