氯氰菊酯通过调控miR-409b对青春期鼠雄性生殖系统的影响及作用机制

徐玮 邵颖颖 杜二球

[摘要] 目的 探討氯氰菊酯对青春期雄性大鼠生殖系统的影响及相关机制。 方法 随机将40只SD健康青春期雄性大鼠分为正常组和低、中、高剂量组各10只，四组大鼠分别进行干预。采用RT-PCR法检测miR-409b、miR-30b-3p表达，检测各组大鼠生殖系统重量，检测精子生成、存活情况，采用免疫透射比浊法检测性激素、氧化应激指标水平。 结果 高剂量组miR-409b、miR-30b-3p表达、精囊重量高于低、中剂量组（P<0.05），前列腺、睾丸、附睾重量及精子数量、每日精子生成量、精子成活率低于低、中剂量组（P<0.05）。高剂量组T、LH水平低于低、中剂量组（P<0.05），FSH、LPO、TAS、ROS水平高于低、中剂量组（P<0.05）。 结论 氯氰菊酯干预能够调控青春期雄性大鼠miR-409b、miR-30b-3p表达，影响大鼠生殖系统发育、精子生成、存活及性激素水平，其机制可能是氯氰菊酯干预能够引起睾丸氧化应激反应。

[关键词] 氯氰菊酯;生殖系统;氧化应激;睾丸;性激素;精囊;精子生成

[中图分类号] R392.5          [文献标识码] A          [文章编号] 1673-9701（2021）28-0027-04

Effect of cypermethrin on reproductive system of adolescent male rats through regulation of miR-409b and mechanisms

XU Wei1   SHAO Yingying2   DU Erqiu1

1.Department of Obstetrics and Gynecology， Taizhou Central Hospital in Zhejiang Province， Taizhou University Hospital， Taizhou 318000， China; 2.Department of Gastroenterology， Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University， Taizhou   317000， China

[Abstract] Objective To explore the effect of cypermethrin on reproductive system of adolescent male rats and related mechanisms. Methods A total of 40 healthy male SD rats were randomly divided into the normal group， the low-dose group， the medium-dose group， and the high-dose group， with 10 rats in each group. The four groups were intervened separately. The RT-PCR method was used to detect the expression of miR-409b and miR-30b-3p. The weights of reproductive system of rats in each group were collected. The spermatogenesis and survival status were recorded. The immunoturbidimetric method was used to detect the levels of sex hormones and oxidative stress indicators. Results The expression of miR-409b and miR-30b-3p and weight of seminal vesicles in the high-dose group were higher than those in the low-and medium-dose groups（P<0.05）. The weight of prostate， testes and epididymis， number of sperm， daily spermatogenesis， and sperm survival rate in the high-dose group were lower than those in the low-and medium-dose groups（P<0.05）. The levels of T and LH in the high-dose group were lower than those in the low-and medium-dose groups（P<0.05）， while the levels of FSH， LPO， TAS， and ROS in the high-dose group were higher than those in the low-and medium-dose groups （P<0.05）. Conclusion Cypermethrin intervention can regulate expression of miR-409b and miR-30b-3p in adolescent male rats， and affect development of reproductive system， spermatogenesis， survival， and sex hormone levels. The mechanism may be that cypermethrin intervention can cause testicular oxidative stress.

[Key words] Cypermethrin; Reproductive system; Oxidative stress; Testes; Sex hormones; Seminal vesicles; Spermatogenesis

據不完全统计，我国农业不断发展，农药应用范围较为广泛，由农药引起的人体健康问题日益增多，引起广大专家学者的关注[1-2]。现阶段农药污染已成为我国主要的环境污染源之一，长期生活于农药污染环境中会对人体健康造成一定的影响，主要表现为农药对人体生殖系统、神经系统、免疫系统的危害。有研究表明，长期、大量氯氰菊酯暴露会对机体造成一定的危害[3]。氯氰菊酯为我国常用的菊酯类农药，应用范围包含林业、农业、公共卫生等各个领域，具有高效、毒性较小的特点。但是，近年来有专家在空气、土壤中发现大量的氯氰菊酯残留[4-5]。基于此，有学者设计动物实验探讨氯氰菊酯的毒害作用发现，氯氰菊酯可能对雄性生殖系统造成一定的影响，但其具体作用机制尚未研究透彻[6]。本研究使用氯氰菊酯对青春期雄性大鼠进行干预，探讨其机制，现报道如下。

1 材料与方……