Omicron variant and change of electrostatic interactions between receptor binding domain of severe acute respiratory syndrome coronavirus 2 with the angiotensin-converting enzyme 2 receptor

Rujittika Mungmunpuntipantip, Viroj Wiwanitkit

Rujittika Mungmunpuntipantip, Consultant, Private Consultant, Bangkok 102002022, Thailand

Viroj Wiwanitkit, Department of Community Medicine, Dr. DY Patil University, Pune 310330,India

Abstract BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are currently a new hazard. Since the first appearance of classical SARS-CoV-2 in late 2019, pathogen genetic alterations have continued to occur, and some new hazardous forms have already emerged. The underlying pathophysiological process leading to clinical issue is molecular change caused by genetic mutation.AIM To determine the change in the interaction between receptor binding domain of omicron variant SARS-CoV-2 and the angiotensin-converting enzyme 2 (ACE2).METHODS The researchers investigated how alterations in the binding area of the SARS receptor CoV2 interacted electrostatically with the ACE2 receptor. In this report, three important coronavirus disease 2019 variants, beta, delta, and omicron, were investigated.RESULTS According to this study, there was a change of electrostatic interactions between the receptor binding domain of SARS-CoV-2 with the ACE2 receptor due to each studied variant. The most change was detected in omicron variant followed by delta variant and beta variant.CONCLUSION Our results may support the clinical finding that the omicron variant is more transmissible than the wild type and other variants.

Key Words: Omicron; COVID-19; SARS-CoV-2; ACE2; Electrostatic

INTRODUCTION

In late 2019, a novel coronavirus epidemic emerged in Asia and quickly spread throughout the world[1]. A pandemic occurred, resulting in millions of cases of coronavirus disease 2019 (COVID-19) all across the world. The disease has already infected over 200 million individuals worldwide, resulting in millions of deaths. Since the initial appearance of classical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019, scientists have been keeping a tight eye on the pathogen's genetic mutations all across the world[2]. Several pathogenic genetic mutations have been identified, and several variants have already proven to be troublesome novel variants[2,3].

The delta variant is one of the dangerous mutations that has spread globally[4,5]. Because transmission of the delta variation is higher than that of COVID-19, it can provide a concern in disease control. A newer form, the delta plus variant, has also been discovered, and it is now being considered in clinical practice[6,7]. The impact of novel variations on disease epidemiology and clinical characteristics is interesting. The newest troublesome variant of concern, the omicron variant, was discovered in Africa in November 2021[8]. There are various structural alterations in this new variant molecule. Omicron is spreading in a rapid manner, and many nations have already reported cases[9].

Clinically, the underlying pathophysiological mechanism that can result in a clinical disease is molecular change caused by genetic mutation. The impact of molecular changes is interesting, but it has received little research. The clinical impact of the omicron mutation is unknown. Pathogenesis may change as a result of molecular changes. A change in the interaction between receptor binding domain of SARS-CoV-2 with the ACE2 is an interesting issue. The authors conducted this study to see how mutations are associated with electrostatic interactions between the receptor binding domain of SARSCoV-2 and the ACE2 receptor. In this report, three important COVID-19 variants, beta, delta, and omicron, are investigated.

MATERIALS AND METHODS

The current research is in the field of medical molecular bioinformatics. It is part of a series of experiments aimed at determining the effects of molecular changes in mutants of SARS-CoV-2. The goal of this research is to see how electrostatic interactions between SARS-CoV-2 and ACE2 receptor change according to the emerging variants. For the investigation of change of electrostatic interactions between receptor binding domain of SARS-CoV-2 with the ACE2 receptor, the authors applied a conventional informatics technique, as described in a recent publication[10].

Various protein-protein interactions are known to be dominated by electrostatic interactions[11]. Analysis was performed according to the published protocol[10]. Briefly, we examined the impact of electrostatic interactions on binding energetics. At the molecular level, both molecular mechanics and Monte Carlo simulations were used to assess the interaction between the receptor binding domain of spike viral protein and ACE2. The protein structure was obtained from the protein data bank and used in all computations (PDB ID: 6m17). To begin, the crystal structure was optimized using the pythonbased open technique[12]. Then, using multiconformation continuum electrostatics[13], rotamers were created, with each rotatable bond rotated by 60 degrees to sample precisely the sidechain conformations. Finally, the Poisson Boltzmann equation was utilized to calculate electrostatic interactions using optimized protein structures with the most occupied conformers[10]. When DELPHI was used to calculate pairwise electrostatic interactions between conformers, it is referred to as DELPHI[10]. The Boltzmann distribution for all conformers was then estimated using Monte Carlo sampling for the WT and altered structures at pH 7 using multiconformation continuum electrostatics. For single and double mutant structures, as well as the wild type, the electrostatic and van der Waals contributions to the interaction energies of SARS-CoV-2/ACE2 were estimated[10].

The research type of SARS-CoV-2 included both wild type and mutation-free SARS-CoV-2.In silicomutation assignment was by PyMol (PyMol, version 2.4). The variants studied are: (1) Beta (K417N, E484K, and N501Y assigned mutations); (2) Delta (T478K, P681R, and L452R assigned mutations); and (3) Omicron (K417N, E484K, and N501Y assigned mutations) (A67V, T95I, G142D, L212I, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, and L981F assigned mutations).

The overall electrostatic interactions value for wild type was derived from the previously mentioned bioinformatic procedure. The already described molecular changes were used for simulation to get the overall electrostatic interactions value for each specific variant. We then calculated the effects of the aforementioned mutations and compared our findings to those of the wild type (native) protein. In brief, the effect of variant on electrostatic interactions was calculated based on a direct comparison to the baseline electrostatic interactions value in wild type. For calculation, the derived overall electrostatic interactions for wild type and each SARS-CoV-2 variant were used as basic parameters. For each type, the change of electrostatic interactions compared to wild type was calculated by the formula “change of electrostatic interactions comparing to wild type = 100 x (electrostatic interactions in that type/ electrostatic interactions of wild type)” and presented in percentage.

RESULTS

The electrostatic interactions between the receptor binding domain of SARS-CoV-2 with the ACE2 receptor for wild type, beta variant, delta variant, and omicron variant SARS-CoV-2 are presented in Figure 1. The values are equal to -39.38, -41.26, -163.82, and -643.71 kcal/mol, respectively.

Figure 1 Graphical result showing electrostatic interactions between receptor binding domain of severe acute respiratory syndrome coronavirus 2 and the angiotensin-converting enzyme 2 receptor.

There were differences in electrostatic interactions between the receptor binding domain of SARSCoV-2 with the ACE2 receptor among the variants studied. The most change was detected in the omicron variant, followed by delta variant and beta variant (Table 1).

Table 1 Change of electrostatic interactions between receptor binding domain of severe acute respiratory syndrome coronavirus 2 and the angiotensin-converting enzyme 2 receptor

DISCUSSION

In clinical genetics, a genetic change may occur, which may result in a new clinical condition. The clinical problem caused by the pathogen's genetic variation has already been noticed in COVID-19[4,5]. In clinical virology, a mutation in the SARS-CoV-2 virus could occur, and the new variety could be clinically significant. SARS-CoV-2 variations have been reported in a number of places. The changes occur at the receptor-binding region of the spike glycoprotein, which is critical for binding to the ACE2 receptor. The interaction between receptor and SARS-CoV-2 is a significant factor of sickness, according to pathophysiology.

Basically, several alterations have been discovered in the omicron variant's molecular structure. The mutations could lead to a shift in molecular pathogenesis. A key feature, electrostatic interaction with receptor, was evaluated in this study. The ability of SARS-CoV-2 to bind to a receptor is a critical factor in its transmission. There is no doubt that the new variant spreads quickly[7], which can be explained by the change in electrostatic interactions between receptor and SARS-CoV-2.

As a result, measuring changes in virus-receptor electrostatic interactions can help researchers better understand disease pathogenesis. According to this study, there has been a significant change in electrostatic interactions. The change of electrostatic interaction has been well described in the delta variant[10], and a change was also observed in the omicron variant. In delta variant, a replacement due to mutation resulted in electrostatic interaction change, and the increased magnitude of electrostatic interactions corresponded to the increased transmissibility of the virus[14].

According to this study, there is a different change of electrostatic interactions between receptor binding domain of SARS-CoV-2 and the ACE2 receptor due to different SARS-CoV-2 variants. The most change was detected in omicron variant, followed by delta variant and beta variant. According to Table 1, the greatest percentage of change compared to wild type was detected in omicron variant. The greatest degree of change indicates the most changes in electrostatic interactions, which can also indicate major changes in clinical features. When compared to wild type, the omicron variant poses around 16 times more electrostatic interactions, implying a significantly stronger connection between the virus and its receptor.

This finding can support the clinical observation that the omicron variant has an increased transmissibility compared to the wild type and other variants. The data from this preliminary study are useful for explaining the pathogenesis of the omicron variant. Further studies on the detailed flexibility of molecular binding, molecular mass change, and immunological epitope change will add to our understanding of the virological properties of the variant.

CONCLUSION

Each studied variant affects the electrostatic interactions between the SARS-CoV-2 receptor binding domain and the ACE2 receptor, according to this study. The omicron form demonstrated the greatest change, followed by the delta and beta variants. These results could support the clinical finding that the omicron variant is more contagious than the wild type and other SARS-CoV-2 variants.

ARTICLE HIGHLIGHTS

Research background

According to this study, each investigated variant altered the electrostatic interactions between the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain and the angiotensin-converting enzyme 2 (ACE2) receptor. The omicron variant showed the biggest alteration,followed by the delta and beta variants. This finding could back up the clinical observation that the omicron variant is more transmissible than the wild type and other SARS-CoV-2 variants.

Research motivation

Each studied variant affected the electrostatic interactions between the SARS-CoV-2 receptor binding domain and the ACE2 receptor. The omicron form, followed by the delta and beta variants, displays the most change. This could support the clinical finding that the omicron variant is more contagious than the wild type and other SARS-CoV-2 variants.

Research objectives

The authors conducted a study to see how mutations are associated with alterations of electrostatic interactions between receptor binding domain of SARS-CoV-2 with the ACE2 receptor.

Research methods

The researchers investigated how mutations affect electrostatic interactions between the SARS-CoV-2 receptor binding domain and the ACE2 receptor. In this report, three important coronavirus disease 2019 variants, beta, delta, and omicron, were investigated.

Research results

There was a change of electrostatic interactions between the receptor binding domain of SARS-CoV-2 with the ACE2 receptor due to each studied variant compared to wild type. The most change was detected for the omicron variant, followed by delta variant and beta variant.

Research conclusions

Our findings can support the clinical observation that the omicron variant has an increased transmissibility comparable to the wild type and other variants.

Research perspectives

Our findings are consistent with the clinical observation that the omicron variation is more transmissible than the wild type and other variants.

FOOTNOTES

Author contributions:Mungmunpuntipantip R and Wiwanitkit V contributed to study conception and design, acquisition of data, and analysis and interpretation of data; Mungmunpuntipantip R drafted the article, revised it critically for important intellectual content, and approved the version of the article to be published

Conflict-of-interest statement:All authors declare that there are no conflicts of interest.

Open-Access:This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Country/Territory of origin:Thailand

ORCID number:Rujittika Mungmunpuntipantip 0000-0003-0078-7897; Viroj Wiwanitkit 0000-0003-1039-3728.

S-Editor:Ma YJ

L-Editor:Filipodia

P-Editor:Ma YJ