Differential Expression of Iron Deficiency Responsive Rice Genes in Low Phosphorus and Iron Toxicity Conditions and Association of OsIRO3 with Yield under Acidic Soils

Differential Expression of Iron Deficiency Responsive Rice Genes in Low Phosphorus and Iron Toxicity Conditions and Association ofwith Yield under Acidic Soils

SUPPLEMENTAL DATA

Fig. S1. Flow chart depicting schematic view of experimental plan.

Fig. S2. Root morphology and leaf bronzing of rice plants grown in normal (0.0284 mmol/L) and high Fe2+(3.6000 mmol/L)concentrations at 10 d after treatment.

SH, Shasharang.

Fig. S3. Nucleotide polymorphism across OsIRO2.

A, Gene overview of OsIRO2 in the 3′-5′ direction, showing the location and size of exons (dark shaded boxes) and introns (lines).

B, SNP Seek Database (snp-seek.irri.org) was used in survey polymorphism across 3 024 genotypes and data summarized showing the position of the non-synonymous (red), synonymous SNPs (orange) and InDels (blue) for the gene in five rice genotypes with contrasting response to Fe toxicity.

C, Allelic frequency of the non-synonymous SNPs observed only in tolerant genotypes (located at positions 41 972 116 and 41 972 720 bp of rice chromosome 1) for different sub-populations.

SNP, Single nucleotide polymorphism; InDel, Insertion-deletion.

Fig. S4. Nucleotide polymorphism across

A, Gene overview ofin the 3′-5′ direction, showing the location and size of exons (dark shaded boxes) and introns (lines).

B, SNP Seek Database (snp-seek.irri.org) was used in survey polymorphism across 3 024 genotypes and data summarized showing the position of the non-synonymous (red), synonymous SNPs (orange) and InDels (blue) for the gene in five rice genotypes with contrasting response to Fe toxicity.

C, Allelic frequency of the non-synonymous SNPs observed only in the tolerant genotypes located at positions 6 134 135 and 6 136 209 bp of rice chromosome 3 for different sub-populations.

SNP, Single nucleotide polymorphism; InDel, Insertion-deletion.

Fig. S5. Nucleotide polymorphism across

A, Gene overview ofin the 3′-5′ direction, showing the location and size of exons (dark shaded boxes) and introns (lines).

B, SNP Seek Database (snp-seek.irri.org) was used in survey polymorphism across 3024 genotypes and data summarized showing the position of the non-synonymous (red) and synonymous SNPs (orange) for the gene in five genotypes with contrasting response to Fe toxicity.

C, A novel SNP occurring at the 272 bp position of the gene discovered by sequencing contrasting genotypes.

D, Allelic frequency of the non-synonymous SNP located at position 27 187 655 bp of rice chromosome 3 observed only in the tolerant genotypes for different sub-populations.

SNP, Single nucleotide polymorphism; InDel, Insertion-deletion.

Fig. S6. Nucleotide polymorphism across

A, Gene overview ofin the 3′-5′ direction, showing the location and size of exons (dark shaded boxes) and introns (lines).

B, SNP Seek Database (snp-seek.irri.org) was used in survey polymorphism across 3 024 genotypes and data summarized showing the position of the non-synonymous (red), synonymous SNPs (orange) and InDels (blue) for the gene in five rice genotypes with contrasting response to Fe toxicity.

C, Allelic frequency of the non-synonymous SNP observed only in tolerant genotypes located at position 27 187 655 bp of rice chromosome 4 for different sub-populations.

SNP, Single nucleotide polymorphism; InDel, Insertion-deletion.

Fig. S7. Nucleotide polymorphism across

A, Gene overview ofin the 3′-5′ direction, showing the location and size of exons (dark shaded boxes) and introns (lines).

B, SNP Seek Database (snp-seek.irri.org) was used in survey polymorphism across 3 024 genotypes and data summarized showing the position of the non-synonymous (red), synonymous SNPs (orange) and InDels (blue) for the gene in five rice genotypes with contrasting response to Fe toxicity.

C, Allelic frequency of the non-synonymous SNPs observed only in tolerant genotypes located at position 15 001 469 bp of rice chromosome 3 for different sub-populations.

SNP, Single nucleotide polymorphism; InDel, Insertion-deletion.

Table S1. List of rice genotypes used in this study.

Table S2. List of primers used in this study.

PCR conditions for allele mining/sequencing are as described by Tyagi et al (2020) at 94ºC for 5 min followed by 34 cycles of 1 min at 95 ºC, 1 min at 55 ºC, 2 min at 72 ºC, and a final extension for 5 min at 72 ºC.